FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365141
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT29670.8125628181989971No Hit
GATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAACTCTTA27690.758337190290874No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA22920.6277027230576682No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC21720.5948387061436541No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT19650.5381482769669799No Hit
CATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGC18560.5082967949367505No Hit
ATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAG18480.5061058604758162No Hit
ACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCA17140.4694077082551672No Hit
GTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGAT16440.45023703172199236No Hit
CCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTC16100.44092556026302165No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA15130.41436047992419367No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA14950.40943087738709155No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC14890.40778767654139086No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT14760.40422740804237267No Hit
GTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAG14580.39929780550527055No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA14270.3908079344691503No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT14160.38779539958536563No Hit
TATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCAC13990.38313966385588033No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG13400.3669815222064901No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG13180.3609564524389209No Hit
GTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAG12550.34370284355906344No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC12380.33904710782957814No Hit
GGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTT12310.3371300401762607No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG11990.32836630233252356No Hit
ATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAG11630.3185070972583194No Hit
GGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCA11500.31494682875930124No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11060.3028966892241627No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA11050.3026228224165459No Hit
TTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATC10880.2979670866870606No Hit
GCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTG10550.2889294820357068No Hit
TCATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGT10420.2853692135366886No Hit
CAACAATGCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTG10330.2829044122681375No Hit
ACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAG10040.2749622748472508No Hit
TAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCT9810.2686633382720648No Hit
AGTAGAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACA9470.25935186681309413No Hit
ATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTC9410.2577086659673934No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC9240.2530529302379081No Hit
GATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGT9070.24839719450842276No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG8890.24346759197132065No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA8800.24100279070276964No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC8800.24100279070276964No Hit
TTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTT8650.23689478858851787No Hit
GAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTA8570.2347038541275836No Hit
ACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAAT8500.23278678647426612No Hit
GCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAA8430.23086971882094864No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC8250.22594011628384653No Hit
GTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAAC8250.22594011628384653No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG7960.21799797886295977No Hit
AACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTA7920.21690251163249263No Hit
GCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAGGAACG7690.21060357505730665No Hit
TTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGG7550.20676943975067164No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG7510.20567397252020453No Hit
GTGGTTAGGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAA7440.20375690486688705No Hit
ATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTTCTATGAAGACA7430.20348303805927023No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA7390.20238757082880313No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA7380.20211370402118634No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA7170.19636250106123387No Hit
GTAGAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACAT7130.19526703383076674No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT7040.1928022325622157No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA7000.19170676533174855No Hit
TGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATG7000.19170676533174855No Hit
GTCAATAACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTA6820.18677716279464643No Hit
ATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATTTGGCC6720.1840384947184786No Hit
GATGAGTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACT6690.18321689429562826No Hit
GAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTC6690.18321689429562826No Hit
TCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCA6650.18212142706516113No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC6640.18184756025754434No Hit
ATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTT6630.18157369344992755No Hit
GATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGT6560.17965662579661007No Hit
GAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAAATCAGC6550.17938275898899328No Hit
ATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCA6520.17856115856614294No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT6390.17500089006712477No Hit
GTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAG6380.17472702325950798No Hit
GGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGA6240.170892887952873No Hit
GTACAAATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGA6230.1706190211452562No Hit
ATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAA6200.1697974207224059No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT6090.16678488583862122No Hit
ATGCAAATCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCA5900.16158141649390234No Hit
TCTCTGTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGAT5890.16130754968628558No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA5880.1610336828786688No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT5820.15939048203296807No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG5800.15884274841773452No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG5800.15884274841773452No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA5780.15829501480250094No Hit
GGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAA5520.15117447780446458No Hit
GCATTACCTCATCCCCAGTTTCAGTCAATAACCAACCCTGTCCTATCCTT5500.150626744189231No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG5480.15007901057399745No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC5480.15007901057399745No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT5420.14843580972829673No Hit
GAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAAT5290.14487554122927856No Hit
ACCTGAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACA5280.14460167442166177No Hit
GAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACAATTAATCCCA5230.14323234038357785No Hit
TTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGC5200.1424107399607275No Hit
ATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCA5120.14021980549979324No Hit
TACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCATGAT5030.13775500423124218No Hit
ACATAACTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTC4980.13638567019315825No Hit
AAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCA4960.1358379365779247No Hit
GTTAGGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAA4890.1339208689246072No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA4880.1336470021169904No Hit
ACATATTCCCTATTCTTTCTCTCTGAAACTTCTGTCAGTTGGCTGTTTTA4870.13337313530937364No Hit
CTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCAGAGAGT4870.13337313530937364No Hit
GTCCTATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTA4830.1322776680789065No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG4750.13008673361797224No Hit
GAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTAT4740.12981286681035545No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA4720.1292651331951219No Hit
ATCACGACCTGAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTT4700.12871739957988831No Hit
AATCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTC4660.12762193234942118No Hit
CAATTAATTTGGCCCATTCACAAACATAACTCTTAATTAGGACAGAGAAA4640.12707419873418763No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA4630.12680033192657084No Hit
ACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAACCAACCCTGTCC4610.12625259831133726No Hit
GGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTTCTATGAA4480.12269232981231909No Hit
GTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGCA4410.12077526215900158No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG4390.12022752854376803No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA4320.11831046089045053No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA4300.11776272727521697No Hit
ACCCAATTTTCTCTGTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAA4270.11694112685236661No Hit
GTACCATTATCAATTAATTTGGCCCATTCACAAACATAACTCTTAATTAG4210.11529792600666591No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT4200.11502405919904914No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA4180.11447632558381556No Hit
AATTTGGCCCATTCACAAACATAACTCTTAATTAGGACAGAGAAAATTGG4160.113928591968582No Hit
ACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAACC4130.11310699154573164No Hit
ACAATGCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGG4100.1122853911228813No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC4100.1122853911228813No Hit
GGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTG4090.11201152431526452No Hit
AATTAATTGATAATGGTACAAATCAGCACAGAGTTTTCCAAGGATAGGAC4060.11118992389241417No Hit
ACTCTGTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAAC4040.11064219027718059No Hit
GGATAGGACAGGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTC3950.10817738900862955No Hit
TGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGC3900.10680805497054562No Hit
ATAACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCAT3850.1054387209324617No Hit
CTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTAAGA3810.10434325370199458No Hit
TTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAG3800.1040693868943778No Hit
TCATAGAACAATTAATCCCAATTATACCTAACCACAGACTGGAATTTCCT3740.10242618604867709No Hit
GGTAAGTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGAC3740.10242618604867709No Hit
GATATAGAGTGGTAATAAGGGAGGAAGGTATGAATATAGTTAGTGACCCA3730.1021523192410603No Hit
CGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTT3710.10160458562582673No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT3690.10105685201059317No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT3680.10078298520297638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA100.0070746867145.098251
GCTAGGC151.229408E-4144.99898
GACGCGT151.229408E-4144.99896
ACGCGTG151.229408E-4144.99897
CGCGTGC203.8641857E-4108.7491768
CTATATA406.085047E-790.686411
CGTAGAA259.3720166E-487.023182
TACTGTA259.3822234E-486.9993447
GCGTGCA259.3822234E-486.9993449
AGTAGAA5200.079.525011
AGCGAAA851.8189894E-1276.816731
CTCCGAC300.001934812972.49945145
AGGGCTA300.001934812972.499455
TAGTAGA1400.067.330143
AAGATGT3250.066.922576
TAAGATG3400.066.102445
GCGAAAG1009.094947E-1265.267382
GATAATA451.1888538E-464.488121
AGATGTA3600.062.4300887
GGGACGC350.00356479762.1423874
Produced by FastQC (version 0.11.5)