FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701017
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG7120.1015667237741738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1650.065.9502941
GCGAAAG2300.047.2986642
CGAAAGC2500.046.4157563
TATATAC7550.046.1083643
CGTACAC700.001054661541.442643
CAGCTCG5250.041.4278569
TATACAG7150.034.487235
GGTCGTT2551.8189894E-1234.119497
TATACCA1956.9630914E-933.47295
TACTCCG950.004718499330.5366785
AGGTCGT3100.030.413556
CGTATAC1050.007694146627.6284263
TATACTT6200.025.7345435
ATATACT4800.025.68584
ACAGCTC9050.025.6349168
GTATAGA3151.02591E-925.3332881
ATATACA9750.025.2906344
TGTATAT8900.025.2685951
GTATTAT5750.025.2331561
TATAATA1751.2523116E-424.8655832
Produced by FastQC (version 0.11.5)