FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916850
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC14040.153133009761684No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT12990.14168075475813927No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT12730.13884495828107107No Hit
GGTCAGGGCGGTTGGGATACGCAAAAGAGACGTCTTGGAATTCCACCAGC12700.1385177509952555No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC12280.1339368489938376No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC10850.11833996836996237No Hit
GTACAAATTCTGCAGCAAGGCAGCCACTGTGCTCTTCCCGGACCCATTGG10610.11572231008343786No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC10450.11397720455908818No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC9230.10067077493592191No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG9230.10067077493592191No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG100.0070875133145.022265
CGGTTCG2950.051.634991
CGAAAGC1950.044.624673
AGCGAAA2000.043.520921
TGGTGCG4050.042.962538
GTAGAAA5150.040.8448751
GCGAAAG2150.040.4735342
TTCGCAG3800.040.0719384
CAAGACG8350.039.939716145
GTTCGCA3850.039.553683
GGTGCGG4450.039.1007279
GGTTCGC3900.039.046582
TCGCAGT4500.033.8385245
GCATTAA6050.032.370931
CGCAGTT6300.032.227176
AGCTATC4250.030.7055729
CATTAAG5750.029.0060332
TATACTC2104.9790106E-727.6232855
ACCGTGC5900.027.0336258
TTACGGG10200.024.877197145
Produced by FastQC (version 0.11.5)