FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164980
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA104590.8977836529382478No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT67630.5805249875534344No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT61390.5269618362546996No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT50560.4339988669333379No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC39490.33897577640817866No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG36370.3121942007588113No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC35760.30695805936582604No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG35040.3007776957544336No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33250.28541262510944393No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG32760.2812065443183574No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG32760.2812065443183574No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA29680.2547683222029563No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG29570.2538240999845491No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC28120.24137753437827258No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG28020.24051915054335696No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG27350.2347679788494223No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA26310.22584078696629983No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC25850.221892221325688No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG24600.21116242338924274No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT23730.20369448402547682No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT23660.20309361534103593No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC22910.19665573657916877No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG22810.1957973527442531No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG22790.19562567597727No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA22610.19408058507442189No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG21860.18764270631255472No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG21200.18197737300211161No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20870.1791447063468901No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20660.17734210029356728No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20520.1761403629246854No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT19710.16918745386186887No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA19560.16789987810949544No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA19430.16678397912410514No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG19330.16592559528918951No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA18950.16266373671651016No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG18420.15811430239145735No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG17990.1544232519013202No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA17750.1523631306975227No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA17400.14935878727531804No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT17210.14772785798897836No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG16880.1448951913337568No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT16650.14292090851345088No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA16210.13914401963982215No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC16150.13862898933887277No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG16020.13751309035348247No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT15920.13665470651856684No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA15620.13407955501382No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA15560.1335645247128706No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT15540.1333928479458875No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT15520.13322117117890436No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT15460.132706140877955No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT15390.13210527219351403No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT15010.12884341362083468No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG14930.1281567065529022No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14700.12618242373259625No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA14240.12223385809198441No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC14180.12171882779103504No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA14160.12154715102405192No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT13980.1200020601212038No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA13830.11871448436883036No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC13800.11845696921835569No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT13650.11716939346598225No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC13600.11674020154852445No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC13590.11665436316503287No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG13550.11631100963106662No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT13510.11596765609710037No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA13480.1157101409466257No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG13430.11528094902916788No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA13340.11450840357774383No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA13310.11425088842726913No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC13310.11425088842726913No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC13250.11373585812631977No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA13030.1118474136895054No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT12990.11150406015553915No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT12910.11081735308760665No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG12660.1086713935003176No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT12600.10815636319936822No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC12580.1079846864323851No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA12570.10789884804889355No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT12390.10635375714604543No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA12380.10626791876255386No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12370.10618208037906231No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG12290.10549537331112979No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT12270.10532369654414667No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA12110.10395028240828168No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA12050.1034352521073323No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT11930.10240519150543356No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG11830.10154680767051796No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT11790.10120345413655171No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA11790.10120345413655171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA202.1232172E-6145.10331
GAACGCA100.0070759286145.10331
AACGCAC100.0070822914145.05972
GCCGGGC100.00708411145.047243
CTCATAC100.00708411145.047243
AGTAGAA10250.0120.329571
CCACGGA203.859894E-4108.794772
CGATCCG553.6379788E-12105.488895
GCAGGTA1400.098.433362
CAGGTAC1550.088.899913
GTAGAAA14200.087.342282
CCACGAC353.437818E-582.855675145
AGCGAAA1500.077.388431
CACTCCG300.001935959272.49872145
CGAAAGC1650.070.325933
CAATCCC1050.069.046394145
AGCAGGC2800.067.343363
GAAAGCA1750.066.3073044
TATATTC7000.065.2712553
GACAGTA2200.062.6152157
Produced by FastQC (version 0.11.5)