FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970149
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12760.13152618824531076No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA10810.11142618298838633No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10630.10957079788774715No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10250.10565387378639778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG151.2295395E-4145.013465
ACGGGCG203.8661802E-4108.74888145
GTCGCAT400.00604737854.3828473
AGCGAAA1950.052.0775831
GTACGGG752.54292E-548.3577581
CAATTCG2650.046.5089539
CGAAAGC2150.043.8435362
AGTAGAA5400.040.2981341
TACCTAT5150.036.6015627
TAATACC5850.035.9435044
ACCTATC5350.035.2332848
ATACCTA5600.034.95866
GTAGAAA7300.034.777841
GCGAAAG2950.034.4117432
GTATTAC6500.033.4784471
TATACGT1551.4638863E-632.7449724
CCTTATA4750.032.057262
CCTTAGA7950.031.9228462
GTAATAC6600.031.8606593
GACAGTA3300.030.7572577
Produced by FastQC (version 0.11.5)