FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045242
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA33420.3197345686453472No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC18140.17354832660761815No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG15920.15230922599742452No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC14480.13853251208810974No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG14480.13853251208810974No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG13760.13164415513345235No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG12760.1220769926964282No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT11780.11270117350814451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG6800.036.2511338
TAAGACC5150.033.797174
CGATAGC900.003628598532.22169145
GTTATAC2506.91216E-1131.9116883
AGCGAAA3450.031.5363041
AGTGATC20400.030.2092828
CCATCGG7950.030.0938429
ATGAACG6000.029.0092356
AAGTGAT21650.028.4664577
GTGATCA21800.028.2678839
GAACGTC6450.028.1016568
CGAAAGC3950.027.541733
GCGAAAG3950.027.541732
GACCATC10050.026.6936577
CGTTATA1658.397979E-526.3732952
ATAGACC2507.8036464E-826.1095623
GTTTAAG6700.025.982151
GTGTAAG7650.025.6000611
AACGTCA7250.024.9995889
GTAGAAA10500.024.868631
Produced by FastQC (version 0.11.5)