FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459741
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT76830.5263262455462989No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT31420.21524366308817797No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA29010.198733884983706No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT25980.17797677807227447No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC24830.1700986681883978No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG24420.16728995075153744No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA22580.15468497493733477No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA22350.15310935296055944No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT19480.13344833090253683No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA19380.13276327786915623No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG18830.12899548618556306No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA18430.12625527405204076No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG17820.12207645054841922No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC17110.11721257401141709No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT16460.11275972929444333No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA15950.10926595882420238No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC15870.10871791639749792No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT15780.10810136866745539No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15770.10803286336411734No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG14920.1022099125803824No Hit
GACTAACACAAACACACAATTACAAAGTGGTAAGTGGTTTATTTTCAAAT14830.10159336485033989No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG14680.10056578530026902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3950.051.4148751
CGAAAGC3900.050.1987723
TAGGGCG450.00964033248.332939
CAATTCG1900.045.7890979
GTAGAAA14450.044.6735041
GCGAAAG4900.042.913692
GGACAGT29550.041.223586
TCGCATC750.001482671638.6716464
GACAGTG32000.037.836927
CAAGGAC29550.037.5429043
ACAAGGA30000.037.4631582
CGCATCG800.002035634436.254675
CAGTGGG34000.032.624739
GTCGCAT900.00362648632.226373
TAGAAAC20450.030.8474942
ACAGTGG40750.030.2461858
AGTAGAA14100.029.8357331
AGGACAG41100.029.2860095
CCATCGG4250.028.999769
ACCATCG4600.028.3703028
Produced by FastQC (version 0.11.5)