FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399254
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA99700.712522529862341No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC46420.3317482029710117No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG38870.27779087999748436No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG36600.26156794977895365No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG35000.2501332853077426No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT34460.2462740860487088No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG32370.23133755558318933No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC32360.23126608893024428No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT29130.20818236002898685No Hit
GTTATAAACATATGCATACAAGGTTGTGTGAAATTATGTTTATTTCTCTA26780.19138769658689558No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC22500.16079996912640593No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA20450.1461493052726667No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC20290.14500583882554563No Hit
ACTATAGACTTTGTATCAAGAATAGATAACGAACTCTTAAAACCCAACAA19460.13907410663110487No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC19340.13821650679576403No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG18790.13428584088378523No Hit
GCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAA18690.13357117435433452No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG18440.1317845080307078No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC18320.13092690819536695No Hit
GTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAACCTCTGCTCCAAGG18300.13078397488947682No Hit
ACTCTGGATCATGGAGATGACTACTGGAAGGGTCCCGTGTCCCCGGGCTC17760.12692477563044308No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC17730.12671037567160787No Hit
AACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGC17410.12442344277736564No Hit
GTATTTCCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGC17050.12185064327134315No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG16920.12092157678305726No Hit
ATACAAGGTTGTGTGAAATTATGTTTATTTCTCTACGGCAGAAAACAAGA16760.11977811033593615No Hit
TCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAAT16460.11763411074758408No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT15900.11363197818266019No Hit
CTATAAGGAGACCCTGGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACC15690.11213117847081373No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC15500.11077331206485741No Hit
GACTATAGACTTTGTATCAAGAATAGATAACGAACTCTTAAAACCCAACA15010.10727144607054903No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA14860.10619944627637298No Hit
ATTATGTGCAGACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTC14860.10619944627637298No Hit
GTAGAAACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAG14780.10562771305281243No Hit
AGTTGTAGTAGGACTCAAACCTCTGCTCCAAGGAAGGCCCTCCACTGACT14570.10412691334096597No Hit
CTCTACGCCCTGGTCAGATGACGGCTCTGGTGGGGCCCAATGGGTCCGGG14510.10369811342329557No Hit
GAGTACAGATGTAGGGGAGAAAGGGAAGCAGCTGGCTGCGGGACAGAGGC14350.10255464697617445No Hit
GTATAGGTGTCTTAGAGTTTGATGATCTACTCACCTATGGAAGGAATTTG14310.10226878036439417No Hit
GGATCTGGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCC14060.10048211404076743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4050.073.425871
CTATCGA554.062671E-665.908499
GCGAAAG5050.060.307252
CGAAAGC5050.058.8692633
ACGGGCG1206.002665E-1154.374504145
ATGAACG10400.053.6850666
GTAGAAA18200.053.4015351
TAAGATG13550.049.7668085
GCTTATG12550.046.2229772
CACGGTA1109.963878E-846.135944145
TTAAGAT14800.045.5635264
CTAGTGA6700.041.119026145
AAGATGT16750.040.2591176
TAGAAAC25950.038.282022
CTTATGA15350.038.2624473
GCATTAA24200.037.7637831
AGTAGAA21150.037.7226871
ACCATCG14200.037.273458
CCATCGG14500.036.4996649
ACTTTCG1001.3918079E-436.2496729
Produced by FastQC (version 0.11.5)