FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871631
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT12870.14765422523980903No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT10040.11518635752973448No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC9450.1084174381131465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1750.049.739241
TATACTG5200.046.0195055
GCGAAAG2700.042.9845281
GCTATAT2300.040.998831
CGAAAGC2950.039.3304862
AATATAC7150.037.5255473
GTAGAAA5650.035.9472351
ATATACT8600.033.7281764
GTATAGA4850.032.9031071
TAGTACT6300.031.0781064
AGTACTG5650.030.8030785
ATACTGT8050.030.6276996
TACTGTG14050.029.4140687
CTATATA3251.8189894E-1229.0062312
TATAGAC4100.028.2987612
GTACACC1804.6912046E-628.2005023
TAATATA9650.027.80392
TCTAGTA6000.026.5890462
GTATAAT3555.456968E-1226.562621
CATTAAG6650.026.1710362
Produced by FastQC (version 0.11.5)