FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882024
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAAGGAGAGGGAGGGAAAGAAATGGGAAAATAGCATTCTAAAACTC21800.2471588074700915No Hit
ATTATTAACTGTACTGACTTCAAGGCACTTGATTTCCCTTATTGGTTAAT19430.22028879032770085No Hit
GACCCACACAGTGTGAAGTGAGTACCTTCCCTCATTTCCCTCAGCTGTAA18230.20668371835687013No Hit
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC16910.1917181391889563No Hit
CCTCTATGATGCGCAGCAGGGCCTTGACGCCGGGTCTCCACAGGTGGCGC15710.1781130672181256No Hit
CATGTATTCCATCAACATTCTTTGTTTTTGATTAACTGATGAATAAGGAA12260.13899848530198725No Hit
ATTAATACTCTGCTTACCCTGATGTAGAACTAATATGCTTATTTTGTGCC12200.1383182317034457No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA11940.13537046610976572No Hit
TCTTAATAGTTGGTATGAAAGTGCCACATTTTAAGGAGGAAAAGAGAGAG11530.1307220665197319No Hit
GAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGC11300.12811442772532267No Hit
ATACAGGACTGGTGTTCCCATTATTCAAAAAAAGTAAACCAAGAGATGTA11300.12811442772532267No Hit
GTGTGAAGTGAGTACCTTCCCTCATTTCCCTCAGCTGTAATATTACCCTG11300.12811442772532267No Hit
GAGGAAGACAGATAATAGTTTAAAGGAGAGGGAGGGAAAGAAATGGGAAA10010.11348897535667965No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA9970.11303547295765196No Hit
ACATTCTGGAGACCTGGAGGCCCCCGCAGGTCCTGCAGGACTACTACGCG9950.1128087217581381No Hit
GTGTTGAAAGTGTCTGAGCATTTCCTGGGTTTCCTGATGGATCAAAAGCT9870.11190171696008272No Hit
GAATTGGAACAGGGATTGGGGCCGGAATTACAGCAGGAGCTGAAGCTGGA9570.10850044896737504No Hit
GTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGT9550.10827369776786118No Hit
TTTTTATACTGCAAAGTTGTTTTAATAAATTACACAAATCTCCAAGGTTG9240.10475905417539659No Hit
ATTTTAAAGTACCCTCGTTCATTTAGTCCTTACAATAACATGGTAGGATA9010.10215141538098738No Hit
TAACTGTACTGACTTCAAGGCACTTGATTTCCCTTATTGGTTAATTCCAA8970.10169791298195968No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGACAGATGCTTAACCGA8920.10113103498317506No Hit
ATCCCAGGACCCTGGGATCATGACCTGAGCCAAAGACAGATGCTTAACCG8890.1007909081839043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.0108.310451
TGGACGC259.385184E-487.002785145
GCGAAAG5000.076.887342
TATATAT4050.075.221712
TATATAA1400.067.354132
GCTCTAG554.045425E-665.952341
GAGCGAA1009.094947E-1265.292811
AGGATAG502.0052936E-458.028172
ATATATG2800.056.9887243
ATATAGA1203.59978E-948.365041
GATAGCA1950.048.356812
TAGACTA450.00962459548.351335
AATATCG450.0096375448.334885145
ATTCGGA1751.8189894E-1245.6013261
TTCGGAA1751.8189894E-1245.5935672
TTCACCG657.322669E-444.6319965
TCGGAAC1801.8189894E-1244.3245663
ATATAAT2150.043.8560183
TGCACCG1002.7267815E-643.5161975
GTATATA10850.043.4616781
Produced by FastQC (version 0.11.5)