FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949682
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT148961.5685250431196969No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA126621.3332884060138024No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG79570.8378594097813795No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA67730.7131860980833584No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC63630.6700137519717126No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT61620.6488487725364912No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC56460.5945147954789076No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG55170.5809313012145119No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA55130.5805101075939104No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC54440.5732445176385359No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC52910.5571338616505315No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG47980.5052217479114062No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG46480.4894269871388528No Hit
GGATACACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGA45560.4797395338650201No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG45470.4787918482186669No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG45170.47563289606415615No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG42990.4526778437413787No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT42950.45225665012077726No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT42160.4439380761138992No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT39440.4152969099130025No Hit
GGATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC35660.37549411276616806No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA35590.37475702393011556No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT35320.37191396699105594No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG35000.36854441802624455No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC34170.3598046503987651No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG33490.35264435884854084No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC33370.3513807779867366No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA31760.33442773475752935No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA31170.3282151288536584No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC30910.32547737031974916No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCCATGGTGTATCCT30510.32126543411373487No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC29220.30768193984933906No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA28670.3018905275660695No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG28640.30157463235061843No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT28580.30094284191971626No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC28560.3007322451094156No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA28280.2977838897652056No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG27990.2947302360158453No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG26850.2827262178287048No Hit
GGATACACCATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACC26060.27440764382182664No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACATTCGAGAAAT25750.2711433932621657No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT25340.26682615865100107No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA24970.26293011766043795No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA24790.2610347463677315No Hit
CACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCG23510.24755655050848602No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG23340.24576647762092996No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA23300.24534528400032854No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA22970.24187043663036678No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA22780.23986976693251005No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA22650.2385008876655554No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACACATCT22610.238079694044954No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA22250.23428895145954118No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG22210.23386775783893976No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT21570.227128659909317No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG21570.227128659909317No Hit
CCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGT20840.21944187633334106No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA20770.21870478749728856No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20040.2110180039213126No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA19920.2097544230595083No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA19710.2075431565513509No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT19430.2045948012071409No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT19380.20406830918138913No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC18970.19975107457022456No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA18730.19722391284661603No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC18590.19574973517451102No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA18150.1911166053478954No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT18070.19027421810669257No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT17830.18774705638308403No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT17670.18606228190067833No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACATTCGAGA17550.18479870103887405No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG17310.18227153931526552No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT17270.1818503456946641No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA17130.18037616802255912No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC17090.1799549744019577No Hit
CCCAATTACAGCAGACACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGAT16970.17869139354015345No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC16850.17742781267834917No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA16770.1765854254371463No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA16130.16984632750752357No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG16050.1690039402663207No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT15900.1674244641890654No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA15820.16658207694786256No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG15810.1664767785427122No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT15720.16552909289635898No Hit
CCCCAGATGTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC15690.16521319768090792No Hit
CCATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGA15310.1612118582851944No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG15240.1604747694491419No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC15200.16005357582854052No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG15190.15994827742339016No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC15060.1585793981564355No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC15020.1581582045358341No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC14760.15542044600192487No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT14620.15394626832981986No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT14430.15194559863196314No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC14420.15184030022681277No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA14090.14836545285685104No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC14020.14762836402079854No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA13850.14583829113324248No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT13640.14362702462508503No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG13320.14025747566027366No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA13220.1392044916087701No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC13210.13909919320361974No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA13080.1377303139366651No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA12930.1361508378594098No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12690.13362367613580123No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT12440.13099121600704236No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGTCTGCTGTAATTGGGTATC12150.12793756225768205No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT12140.12783226385253169No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG12130.12772696544738135No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATATGGTGTATCCTGTTC12090.1273057718267799No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAT11830.12456801329287068No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC11540.12151435954351035No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11370.11972428665595432No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAGCGCAAAATGCC11350.11951368984565361No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATA11040.11624943928599259No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA11030.11614414088084223No Hit
CATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCT10820.11393287437268475No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATATTCGAGAAATTTTT10790.11361697915723368No Hit
GCCATGGAACAGGAACAGGATACACCATGGACATTCGAGAAATTTTTCCC10540.1109845190284748No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA10520.11077392221817409No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT10390.10940504295121946No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG10380.1092997445460691No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC10340.10887855092546767No Hit
GAACAGGAACAGGATACACCATGGACATTCGAGAAATTTTTCCCTAGCAG10310.10856265571001662No Hit
GAGATACCCAATTACAGCAGACACATCTGGGGTGGAGTCTGCTGTCCTGA10250.10793086527911448No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA10170.10708847803791163No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG10100.10635138920185915No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT10050.10582489717610737No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCG9930.10456131631430311No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA9850.10371892907310026No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG9780.10298184023704778No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA9610.10119176734949172No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT9590.100981170539191No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG9500.10003348489283782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGG100.007085542145.036032
TGTAACC100.0070911217144.99783145
ACGCGCC100.0070911217144.99783145
AGCGAAA3050.0130.818421
GCAGGTA8750.0117.686372
CAGGTAC8900.0116.505433
AGTAGAA26800.0107.192991
GGTACTG9850.0105.999435
GAGCGAA352.7515125E-7103.635361
AGGTACT11150.096.241884
ACTGATC11050.095.134338
GTACTGA11450.091.187286
GCGAAAG4400.090.6475142
AGAGTTA850.085.29285145
AGCAGGC7050.084.338313
CGAAAGC4800.083.08483
TTAGAAA1050.082.85591145
GTAGAAA35650.080.95982
GCAGGCA7500.079.2738344
TACTGAT13250.078.795437
Produced by FastQC (version 0.11.5)