FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436093
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5450.066.556261
GCGAAAG7700.047.094872
TTCGACA1001.3893767E-436.2605254
ACCGTGC2002.237357E-1036.252958
CTAGTAC3650.035.7650533
TATACTG12950.033.0404785
CGAAAGC11000.032.9652633
CGGACAG1000.006066716329.008425
CCGTGCA2553.0267984E-928.4307169
CTATACG1100.00965764326.3722113
GTATTAG4750.025.9639471
CTATAGA8900.024.4538171
GTATATA10400.024.4146271
ATACTGG14600.024.3392586
CGTAGCG600.004706513624.1686348
ATGCCGG2401.5992136E-624.16611145
TCTATAC8950.022.6889423
TATAGGC1952.6091497E-422.3157272
GCGCGCA2953.877667E-722.1181349
ATCTATA6950.021.9143642
Produced by FastQC (version 0.11.5)