FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044174
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC36680.3512824490937334No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC29350.2810834209624066No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25490.2441164020555961No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG23980.2296552107215847No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA23840.22831443801512008No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG21430.2052339935681218No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA19410.1858885588034178No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG19280.18464355557598636No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG19080.18272816599532263No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT18710.17918469527109468No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG18290.17516237715170077No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA17980.17219352330167192No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT17610.16865005257744398No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA16940.16223349748222038No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC16620.1591688741531584No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC16430.1573492540515278No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA16210.15524232551279768No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG16170.15485924759666492No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC16140.15457193915956535No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG15830.15160308530953653No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC15450.1479638451062754No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT15140.14499499125624654No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT15050.14413306594494787No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC15020.1438457575078483No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14140.13541804335292779No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC14070.13474765699969546No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG14020.1342688096045295No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA14000.13407727064646313No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA13890.13302380637709807No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG13860.1327364979399985No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13830.13244918950289894No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG13770.1318745726286998No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13570.12995918304803605No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG13480.12909725773673736No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA13140.12584109544960898No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA13020.12469186170121073No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC12910.12363839743184564No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG12900.12354262795281246No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT12870.12325531951571289No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA12750.12210608576731465No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG12660.12124416045601596No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12610.12076531306085No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT12470.11942454035438538No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT12450.11923300139631901No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG12200.11683876442048931No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG12170.11655145598338974No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG12070.11559376119305786No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA12010.11501914431885872No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG11930.11425298848659322No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTCCACCATGTTAC11840.11339106317529454No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11790.1129122157801286No Hit
TAATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC11770.11272067682206223No Hit
GAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATC11740.11243336838496266No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11670.11176298203173034No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11520.11032643984623253No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA11470.1098475924510666No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA11410.10927297557686746No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA11350.10869835870266832No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAA11220.10745335547523689No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT11070.10601681328973905No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10930.10467604058327444No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC10930.10467604058327444No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10920.10458027110424124No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC10580.10132410881711286No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10450.10007910558968142No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC100.007087105145.026024
GCAGGTA4750.0120.606352
CAGGTAC4800.0119.344323
AGTAGAA4550.092.443011
GTACTGA6150.091.9677056
ACTGATC6150.091.95458
TTCGTCT259.3801797E-487.01564
GGTACTG6850.084.686725
AGGTACT7300.080.459634
AGCGAAA809.822543E-1172.519951
GTTGCGC300.001935860172.499115145
ATTATTA754.8548827E-967.66584145
TATATTC5700.063.6078953
GCATTTA350.0035667262.1421145
ATATTCA5850.061.9769254
TAGTAGA1200.060.4275053
CGAAAGC1102.5465852E-1159.331662
GACAGTA5800.058.754917
GTAGAAA7300.058.609212
GCGAAAG1154.0017767E-1156.754741
Produced by FastQC (version 0.11.5)