FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640026
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA39560.24121568804397003No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30200.18414342211647863No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT28510.17383870743512603No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20330.12396144939165597No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG18280.11146164755924601No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA18190.11091287577148166No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC17770.10835194076191475No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG17080.1041446903890548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA11450.070.961211
GTAGAAA13850.058.1302532
CGAAAGC4950.046.8821533
ATGAACG1557.385097E-1042.1075866
GACCGTC700.001056455441.431637
TAGAAAC24450.037.0760883
CTCCGAC1406.613973E-736.24936145
CAATTCG3700.035.269659
CAGGTAC8650.034.3740353
GACAGTA4300.033.723427
CCTTATA20500.033.2585262
AGCGAAA6750.032.2422941
TTATACT21650.032.1560634
TATACGT1153.1548287E-431.5356042
CCATACG1652.3879875E-630.7570329
AGGTACT10650.030.641674
CCCTTAT23900.029.7465531
GGATAGC7050.028.801725
GCGAAAG8100.028.6545722
TATATTC5100.028.439543
Produced by FastQC (version 0.11.5)