FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921692
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTGTCATG55980.6073612443202284No Hit
ATTCTAGGGAGTTGCATGAGAAAAATGAAAACCTAGATCCCAACGAGGCT28200.3059590405471676No Hit
ATAAAAGACAGGCTATCATCATCAGTTGCCCACAACCATGTGCAAATCAC25590.27764155487950426No Hit
GGTATAAACTACGGGCGCAACAGGGCAGAGGAATCTCCAAGACCTCCCGC22690.2461776819154338No Hit
CTACAAACATCACTAAACACACGCACACAAACACCCTTCAACAAAGTAAC18940.20549163928948064No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC18560.20136878697005076No Hit
TCTCAAGGTAATCCAACCTTTCCTTTTCAGTTGAACTGCTGCAAATAACT18280.1983308957873129No Hit
CCCTATACTCTTCCGGGACACTGGGTTGCAAGCCACACCTATGCTCAATT17580.19073616783046832No Hit
GGATAAGAGAAGGAATGTGGGAGCTTGTGTTTGAACTCCATGCTTTTGAC17330.1880237649887381No Hit
ATCAAGGACAGCTATTGATCGCTTGTGTTTTGATTAGATTGGAAAACAGA16980.18422640101031582No Hit
CCCTAATGGTTATCGACATTGCTCCGGAGCAGTAGACCCTATCAAGGACA16720.1814055020549164No Hit
CTTCTAGGAGAGGATGAAGGGAAAAACCCAGAGCAGTGACTGCCTGGGAT16590.1799950525772167No Hit
GTAGTAAACAGGTATAAACTACGGGCGCAACAGGGCAGAGGAATCTCCAA16530.17934407589520143No Hit
AAGCAGGGGCGTGAAGAAAGGCGTGATGGATGTCCTTAGGTTTGTTTACT16220.17598069637145597No Hit
GAGTAGGGGGTGCTGGTATGAAAGACTGTGAGTAGAAAGGAAGATGGGAG15870.17218333239303368No Hit
GAATATAATTATGGAAAATTTCAAACGTATACAAAGTAAACAGAGCAGTA15330.16632454225489643No Hit
AAGCAGGGGTCATTGAAGGCACTCGGCAAGGCAGGGATAAGAGAAGGAAT15260.16556506945921198No Hit
GTGCCAGGAGAGCGGGTCACCGGAGCATGGTGGGTGCTTTTGCTTTCGGT15120.16404612386784306No Hit
GTCTGTGATCTGGTGTTTTTTTTACTCAAACAGCCCATGCGGTGGATCCC14830.160899736571436No Hit
ACATTGATCCGAGCACACACCAGGCCAGCAGATGTGCACATGCGCGTGCA14510.15742786093402134No Hit
ACTTATAACCTTCCAAAGCAGAATTTATTTCTGCAAAAGTGATTTGAGGA14110.153088016387253No Hit
CTCCAAGACCTCCCGCTGTGCCAGGAGAGCGGGTCACCGGAGCATGGTGG13790.14961614074983834No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT13590.14744621847645417No Hit
GAGTAGGGAGGAGGATAATGTGTGGGTCTGCACCCGTGGAAAGGGAAATT13440.14581877677141605No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT13340.14473381563472396No Hit
GCTATTGATCGCTTGTGTTTTGATTAGATTGGAAAACAGATCAACTTCAT13250.14375735061170108No Hit
ATTAAATATCCATAAAAACAGGCTACAATAAAAGACAGGCTATCATCATC12890.13985149051960957No Hit
CCTTTATTAAATATCCATAAAAACAGGCTACAATAAAAGACAGGCTATCA12720.13800705658723306No Hit
GTGTAGAGCCCTGAGTCCTCACTGAAGCCCGAGAGGAAAGATGGATGGTG12650.13724758379154858No Hit
GATCATGACAAGAGGACAATGGTTGTTGGCCACGTGTCTGGCGCTGGGCC12400.1345351809498184No Hit
GAGCAGTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTG12330.13377570815413392No Hit
AGTTTTAACAGTAGCTTTATTCACAGCAGCCTCCAACCCAAACCAGTAAA12060.1308463130850653No Hit
CTTTTAAAGTGACTCATTAACTTCTTATCTTTGTTTTAACTTATAACCTT12050.13073781697139608No Hit
GTATGAAAGACTGTGAGTAGAAAGGAAGATGGGAGGAGAGAGTGTGAAAA12020.13041232863038846No Hit
CTTATAACCTTCCAAAGCAGAATTTATTTCTGCAAAAGTGATTTGAGGAC11560.12542150740160488No Hit
GGTTAGAAGCAAGGGCGATCTCCCAGTCGTTACACCTCTTGAGGTAAGAT11470.124445042378582No Hit
ATTATATTCTTTTTAAGTAAAAGAGCTTTTAAAGTGACTCATTAACTTCT11170.12119015896850575No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG11170.12119015896850575No Hit
CTTCTTATCTTTGTTTTAACTTATAACCTTCCAAAGCAGAATTTATTTCT11140.12086467062749813No Hit
GGATAACATTGATCCGAGCACACACCAGGCCAGCAGATGTGCACATGCGC11090.12032219005915208No Hit
CAATAAAAGACAGGCTATCATCATCAGTTGCCCACAACCATGTGCAAATC11090.12032219005915208No Hit
GAGAAGGAATGTGGGAGCTTGTGTTTGAACTCCATGCTTTTGACACCTGC10810.11728429887641426No Hit
CACTACCACTGTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGA10680.11587384939871453No Hit
GCCTTGGGGAAGCCTGGTCGGAGAGCTGAGTTTCCTCGCGAGCCGGCAAG10610.11511437660303007No Hit
GTGATGGGACGTCAGGAAAACCGTGTGCCAAGTCAAGACCAGACCAGACC10530.11424640769367642No Hit
CATAAAAACAGGCTACAATAAAAGACAGGCTATCATCATCAGTTGCCCAC10460.11348693489799196No Hit
ATGTGTGGGTCTGCACCCGTGGAAAGGGAAATTTTCCATGTCTGTCAATC10430.11316144655698433No Hit
GTATATGTTCTAACAACAAGCAGACTGGTTATTGAAGTGTTACTTTGTTG10380.11261896598863828No Hit
GTTCTATTCTAGGGAGTTGCATGAGAAAAATGAAAACCTAGATCCCAACG10350.11229347764763066No Hit
GCCCACAACCATGTGCAAATCACACTCTACAAACATCACTAAACACACGC10320.11196798930662302No Hit
GTCAAAAGCATGGAGTTCCTTTATTAAATATCCATAAAAACAGGCTACAA10280.11153400485194621No Hit
CTCCTAGAAGTTCGTACTAAATGGAACCACCGAAAGCAAAAGCACCCACC10250.11120851651093859No Hit
TCTTACCACAGAGCGACAACTGAAAGAAATCCAACACAGTTAATGTGCGT9970.10817062532820074No Hit
GTGGTAAGATTTGTACCCTTTTTTAAAAGAGCAGCTTTATCGAGAAGCGA9930.10773664087352391No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC9920.10762814475985472No Hit
CCTAAAAGACTCCTTCATGGCAGTTCTCCACCCCCATCCCAGGCAGTCAC9890.10730265641884708No Hit
GGTCTGACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGG9760.10589220694114737No Hit
AAACAGAGCAGTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACAT9680.1050242380317937No Hit
CAAGAGGACAATGGTTGTTGGCCACGTGTCTGGCGCTGGGCCCAGTGCCG9620.10437326134977847No Hit
AACGTATACAAAGTAAACAGAGCAGTATAACGGACCCCAGGTATCCGGCC9610.10426476523610925No Hit
GATAAAAACCAGCTCGAGAATCTTGACTAGCCTGCTAGCAACTGCAGAGG9540.10350529244042479No Hit
GGAGAAGGGAGAGAGAGAAGCCAGGGAGAGAAGAGAGAGGAAGAGGGAGA9480.10285431575840953No Hit
CTTCTGCAAGGATGGCTATGCCTCCTGCTGAGGTGCTCTTTCTCTCTCGC9390.10187785073538665No Hit
GTAGTGAAGAGAGGAGTGGCAAGTGGATAAAAGAGGAAGAGAAGGAGGGG9380.10176935462171745No Hit
GTGTGGGGGCTGTCTGTGATCTGGTGTTTTTTTTACTCAAACAGCCCATG9320.10111837793970221No Hit
GGAAAAGGGCTCCGTCATTTCTTCCACAAAACTCCTACTCTTGGCTCCGG9310.101009881826033No Hit
CCTTAGGTTTGTTTACTCGCGAAGAAGAATAAATCAAAGTGGGCTCCGCA9240.10025040903034854No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA100.0070909797144.99858145
AGCGAAA7300.0109.304821
CCTAGGC651.5643309E-978.076164145
GCGAAAG10350.077.069122
GGGGCGT3850.067.7989046
TGCGTTA451.1938665E-464.44382145
AGGGGCG4850.059.7997175
GGGCGTG4400.059.3240477
CAAATTA753.7611244E-757.999435145
GGGGTCA4950.054.1972736
CTTGCGG1500.053.1748053
CAAATTT553.2190233E-452.726765145
GGTCATT5200.050.1945468
GCGTGAA5200.050.1918229
TTGCGGA1600.049.848674
GGTAGGT450.00961862648.35911
TCGAAAC450.00963100948.3433532
GGAAATG1950.048.343352
CGAAACC450.00963307448.340733
CCTTGCG1751.8189894E-1245.5808752
Produced by FastQC (version 0.11.5)