FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089615
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC17170.15757859427412435No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT15420.14151787557990667No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT14950.13720442541631678No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC13250.12160258439907674No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG12550.11517829692138967No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG11730.10765270301895623No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11540.10590896784644116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC607.98791E-872.558141
TATTACG300.001932456472.5314943
AGCGAAA2700.061.808791
GTCGTAG502.0023741E-458.0465131
GCTATAT2000.047.1627921
GCGAAAG3850.045.2165152
CGAAAGC4150.041.9459233
CGGACCG907.467791E-540.2915765
GTATAGA2850.033.09671
GTAGAAA8200.032.7291262
GTCTAGT5250.031.7873761
CATTAAG8300.031.4608882
TAGTACT10800.030.8902074
ATATACC3300.030.7681124
TCTAGTA9200.030.7484572
AGTACTG9500.030.5367745
TAAGATG8300.029.708975
AGTAGAA9000.029.0232561
TATATGC1504.3860535E-529.0125963
GTACTGT9500.029.0099336
Produced by FastQC (version 0.11.5)