FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080623
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGTTCTGAGAAATCATACAATAGAAAAACCTTTTTACCTTGGG51150.4733380651716648No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT26590.24606176252032394No Hit
CTTATAAGGAAAGGAATTAAATCTTGCTATTTGGTTAATTTTCACCCTCA26180.24226765486205643No Hit
CTATAATGTTTTCATTGATTTTGTTTTTTGCTATGAGTTCTGTAATTGTT21970.2033086469564316No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC21380.19784883349697352No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG21020.19451742189459229No Hit
ATACATCACTGAATAAAGGTTCTGAGAAATCATACAATAGAAAAACCTTT19010.1759170404479638No Hit
GTATAGAGGCATGAATTTGATGGGAGTTTATTTGGCTCCATTCTTACTAC18980.17563942281443204No Hit
TCTCAAATCTGTGTGAACCATGAAATATCTAAACACATTCTACGGAACTA18540.1715676975226328No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC17650.163331707727857No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC17270.15981521770312127No Hit
CTTTTATATTGGCCATAGCAACTTCTTTCAAGACATGTCTCTAAAGGCAA17180.15898236480252595No Hit
AAAAATACCAGTATCTCAAGAAAGCATGCCACTTAAACAAAAATCTCAAA16210.1500060613183321No Hit
AAATAATACCTGCCATATTGGGGTCTAACTTTTAAAAGTGAGAGCTCTAT16110.14908066920655955No Hit
CTCTTAAAGATTATACATCACTGAATAAAGGTTCTGAGAAATCATACAAT14970.13853119913235237No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA14940.13825358149882058No Hit
GTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAACA14930.1381610422876433No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA14710.1361251796417437No Hit
GCTTAATTGTATACCAATGTGTACAGGAACATCTATGGATAGAGAAAAGT14360.13288630725053974No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC14290.13223853277229894No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT13530.1252055527228275No Hit
ACCTATAGCTTAATTGTATACCAATGTGTACAGGAACATCTATGGATAGA13500.12492793508929571No Hit
GATTATACATCACTGAATAAAGGTTCTGAGAAATCATACAATAGAAAAAC13080.12104128821985094No Hit
ATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGGAG12970.12002335689690115No Hit
TCTATGGATAGAGAAAAGTCTCATTCCATCGAATTCAAATGTGCACTTGG12940.11974573926336937No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA12780.11826511188453327No Hit
CTCTTACATCATACGCAAAGATAAACTCAAAATGGATTAGAGACCTCCAT12670.11724718056158345No Hit
ATGTAGCAGGGTCCCATATGAAGAACTGTATTTTGTATAGAGGCATGAAT12220.11308291605860693No Hit
CAAGAAAGCATGCCACTTAAACAAAAATCTCAAAAATGCACCAAACAGAA11980.11086197499035279No Hit
GTATAGGGTAGAGTGAAAAGAATCAGAGATAGGAACTGTATGCCACTATC11650.10780818102150333No Hit
TCACTGAATAAAGGTTCTGAGAAATCATACAATAGAAAAACCTTTTTACC11570.10706786733208529No Hit
ATTGTATACCAATGTGTACAGGAACATCTATGGATAGAGAAAAGTCTCAT11520.10660517127619899No Hit
AGATTATACATCACTGAATAAAGGTTCTGAGAAATCATACAATAGAAAAA11180.1034588380961723No Hit
TCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAG11110.1028110636179315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5700.086.51871
GCGAAAG6550.075.2771452
TGCGTTA300.001935833772.499535145
TATAAGG5450.070.5106663
TTATAAG6250.063.808452
CTTATAA7400.053.902251
CAGCTCG2800.051.785389
CTATATA6200.051.4679531
CGGGCCA1700.046.9158064
GGTAGGT2300.044.1362762
TACCTAG1053.8221424E-641.4321445
TATTCCA700.001056231241.432145
GTTCTGA22100.040.022379
CTAGTCC1105.263255E-639.5470358
CATCGTA1105.2649284E-639.545204145
CGAAAGC12800.039.08543
GGACATC6350.038.8222436
CTATGGT951.0284237E-438.1611863
GGGTAGG3300.037.3603481
TAAAGGT27250.035.122294
Produced by FastQC (version 0.11.5)