FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091058
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5050.054.5884481
CGAAAGC8150.040.0508272
GCGAAAG10300.033.1031381
GTATTAC15900.029.6568321
GTATAGG4850.028.4197561
GTATATA23900.027.014731
CCTTATA8200.024.768562
TATTACA21500.024.6286722
TTACATC20000.024.2962384
TACATCA20200.024.0556815
TAGTACG2903.278219E-722.5080974
CAATTCG3106.2520667E-721.0479059
TATATAC25850.021.0438943
TATAGAC21850.020.5824382
CAGCTCG11350.020.4400629
TATACAG14550.020.4369035
ATTACAT23850.020.3756683
TTATACT12300.020.0478614
ACATCAA23200.020.0071966
GTATAAG12650.019.4983061
Produced by FastQC (version 0.11.5)