FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184113
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5550.049.6652681
CGAAAGC7600.037.209523
GCGAAAG8150.033.8095172
ACCGTGC10300.028.860248
CTATTGA11850.028.754979
GTCTAGT6850.028.5913941
GAGCTAT13500.026.8552576
GCTATTG13200.025.8153048
GTAGAAA19050.025.511851
TCCACCG13050.023.3362945
GCGATTA1700.003093732521.323792145
CGAGGCG4451.1459633E-1021.180037145
TTAGAGC18900.020.7173863
TAGAGCT20000.019.9400274
AACCGTA2205.905035E-419.7734247
TTAGCAC11050.019.6862053
ACCGTAC2608.0509126E-519.5293061
GTATATA12300.019.4612621
GCATTAA10500.019.3433131
ATAGGAG20900.019.0818023
Produced by FastQC (version 0.11.5)