FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020178
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA32530.31886592339768155No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC18150.17791012940878945No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC13710.13438831262779632No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG13580.133114025199524No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA13110.1285069860357702No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC12440.12193950467467442No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG11250.11027487360048933No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG10850.10635398920580527No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC10290.10086475105324758No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT400.00604762954.382486
AGCGAAA4400.046.163081
CGAAAGC4350.045.006193
CGACTAT657.331683E-444.6215256
GCGAAAG5700.036.892852
TAAGACC6900.031.5260774
GAACGTC3350.030.298228
ACCATCG4650.029.6233758
ATATAGC2701.70985E-1029.5411016
TGAACGT3201.8189894E-1229.4528457
GTAGAAA8550.028.8357892
CCATCGG4600.028.3692979
GTGATCA14350.028.2924169
GTTTAAG8050.027.9354211
AAGTGAT17050.027.2138187
AGTGATC15750.027.1584748
TAGAAAC9700.026.910923
ATGAACG3607.2759576E-1226.1841586
GTATATA10300.026.058771
ACCTCTC8600.025.2904598
Produced by FastQC (version 0.11.5)