FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485582
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA58810.39587178627635505No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC27090.18235277487207033No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG24370.1640434523304671No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21710.1461380119037522No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC20790.13994515280879816No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG20680.13920470226483628No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG18550.1248668871863014No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG18310.12325135872674818No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC18080.12170314395300967No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT17990.1210973207806772No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTATCTCAAATCGGTGCTTC16640.11200997319569031No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC15080.10150903820859433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5600.040.1442832
GTAGAAA15000.040.1391751
AGCGAAA5900.036.8850941
GCGAAAG6600.034.0721361
ATGAACG7250.034.0053376
GTGATCA30450.033.571059
AGTGATC30100.033.4808168
TAAGACC12650.031.5266864
TTAAGAC13350.029.8746093
AGTAGAA15050.029.4018731
AAGTGAT34250.029.2133147
CCATCGG8850.028.671999
TGAACGT8950.028.353547
TAGAAAC21900.027.4842572
ACCATCG8750.027.3434688
GGACAGT18700.027.1432976
TACTGAT13500.026.8533547
CCCTGAA37750.026.702252
GGTACTG13350.026.6146645
GAACGTC9300.026.5059668
Produced by FastQC (version 0.11.5)