FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080445
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAATGGGCGCTGTGCTGCCAACATAGCAGCTGTAGCCGGGACTGT65200.603455057869674No Hit
GTCGTGTAGTGTGCGCGGATTCCACCAGAGAAGGCCTCCAGGATAAAATA47730.44176242196502363No Hit
ATCATATGGAGAGAAATTATCCTATTGTCCGTTTCCAGTGAACACTGCAG39480.36540499516402963No Hit
CACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGC34860.32264483615547296No Hit
CTACACGACAGTCCCGGCTACAGCTGCTATGTTGGCAGCACAGCGCCCAT33710.31200107363169804No Hit
ATATAAACTGTCTGCAAACAATCCACTCTCCCTTGGTTTTCTTGGTCTCC33510.3101499844971285No Hit
GAATAGAAAGAGGGATGTGTTGCAGGAGTGGAGGGGAGAGAAGAGAAAGA27170.25147045893127373No Hit
CACACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTG25320.2343478844365053No Hit
GCTTATAATACTTAATGTATACCAGCCTTTTTACCCCTAATATAAAAAAG25260.2337925576961345No Hit
GGCACATGGAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAA25180.23305212204230663No Hit
GTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGG25010.23147869627792253No Hit
GCCATAGACAGAGCATTCCCTTTTTCCCAGATGGATGTACGCAGAAGAGA24950.23092336953755166No Hit
GATTTACTGCTATCTGAAATAGAGACATTCATCAGGCACATGGAGTACCT23430.21685509211482307No Hit
GATTCATACACGTACAGAAATTAAATATACAGAATTGGATAAAAATGGAG23310.21574443863408133No Hit
ATCTGAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGG22010.20371235925937922No Hit
GTGTGGTGCTGGCAGTGTGCCTCAGTTTACAACTTACAGCTCCCCGTGTT21480.19880697305276995No Hit
ATGTATATGTAAATAGTTTTGTGCTTCATTGCTCTGCTTATGCTTAGTAA21400.1980665373989421No Hit
GAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGGGCCT20540.19010685412029302No Hit
GCTCTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACT20370.1885334283559089No Hit
ATGTATACCAGCCTTTTTACCCCTAATATAAAAAAGCATCTGCTAAATGA20350.18834831944245195No Hit
CAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTGATGA20320.1880706560722665No Hit
ATACAGAATTGGATAAAAATGGAGAAAGCCAGCATTCAAAGTACTGGTTG20170.18668233922133937No Hit
GATATATCATTTATCAGGTACATTCAATTTGTGTTGTTGATGAACATAGA20100.18603445802424No Hit
CATCTGGTTTGACCACATGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAA20010.18520146791368372No Hit
TTACAGGACAAGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCGGTTCT19250.17816732920231942No Hit
GGGCATAGTTGACCCCTTATTACGACATGGTATTAAATGGGCGCTGTGCT18970.17557580441392204No Hit
GTATTTAGGCTACATCTGGTTTGACCACATGCTAGGTGAGGATCCTTTTG18270.1690969924429286No Hit
ATTTAATACCATGTCGTAATAAGGGGTCAACTATGCCCAAAGTATTTGAG18140.1678937845054584No Hit
TTGCAGGAGTGGAGGGGAGAGAATAGAAAGAGGGATGTGTTGCAGGAGTG17510.16206285373156432No Hit
GGCCTACGCGTTCAGTCCCCAAAAAAAGGAAAGTAGTGCTTTATTTGCCA17340.1604894279671802No Hit
CTGCAGAGTGGGACGAAGGCCATAGACAGAGCATTCCCTTTTTCCCAGAT17150.15873089328933912No Hit
CATATGGACTGTGGCAATGTAGAACGTGGATCCTTTACCATTGGGCCTGC17150.15873089328933912No Hit
GATTATTAATGCGTGATGAGGTTGGTGAACTCATGGCACGGGATCTGAGA17080.15808301209223977No Hit
GGACAGGAGAAGTGGCTCGATTAGATTGACTCTCAGAAATCAGATTTTCA17070.15799045763551128No Hit
CCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAAAAAGGAAAGTAGT16770.15521382393365696No Hit
GAGTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGG16600.15364039816927283No Hit
TGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAATCTCAAGTGCTCCCCTG16590.15354784371254437No Hit
GAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAAAAAGGAAA16480.15252974468853112No Hit
GTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTGATGATC16440.1521595268616172No Hit
GTGCTGGCAGTGTGCCTCAGTTTACAACTTACAGCTCCCCGTGTTAGCGG16070.14873501196266353No Hit
ACACTACGTTGTTCAGACGGATGTGTGCTAGCCATTAGCCTTGCACCTCT15880.14697647728482247No Hit
GGAGAAAAGAGAATCAGGAGAAAGCAGATGGATTCATACACGTACAGAAA15290.14151576433784227No Hit
GATCTGGGATGCTGAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATT15260.14123810096765685No Hit
GTGTTGCAGGAGTGGAGGGGAGAGAAGAGAAAGAGAGGGATGTGTTGCAG15170.14040511085710053No Hit
GGCCGACGGGGGAAGAAGGATTTACTGCTATCTGAAATAGAGACATTCAT14980.13864657617925946No Hit
GAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGGAGAGAATAGAAAGAG14760.13661037813123297No Hit
GGAGAAAGCAGATGGATTCATACACGTACAGAAATTAAATATACAGAATT14270.13207520975153758No Hit
ACTTACAGCTCCCCGTGTTAGCGGAGGAGTCGAGAGATTGAGAGAGAGAA14250.1318901008380806No Hit
GGCCATAGACAGAGCATTCCCTTTTTCCCAGATGGATGTACGCAGAAGAG14140.13087200181406736No Hit
ACTTAATGTATACCAGCCTTTTTACCCCTAATATAAAAAAGCATCTGCTA13960.12920602159295475No Hit
GTGTTGCAGGAGTGGAGGGGAGAGGAGGCAGGTATTTAGGCTACATCTGG13930.12892835822276932No Hit
GAATTGGATAAAAATGGAGAAAGCCAGCATTCAAAGTACTGGTTGTAGAA13920.12883580376604084No Hit
GTTTACAACTTACAGCTCCCCGTGTTAGCGGAGGAGTCGAGAGATTGAGA13850.1281879225689415No Hit
GATTCTACTCTGCTTATAATACTTAATGTATACCAGCCTTTTTACCCCTA13770.12744748691511368No Hit
CACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTG13670.1265219423478289No Hit
CCCCTGATAGACTCTTTATTTTATCCTGGAGGCCTTCTCTGGTGGAATCC13610.12596661560745803No Hit
CCTTTAAACCAGAGAAATGGAAACGATTTGAAGTAGAGAATGGTGTTTTA13590.1257815066940011No Hit
GACTAAATGTATATGTAAATAGTTTTGTGCTTCATTGCTCTGCTTATGCT13420.12420808092961697No Hit
CTCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTCCTTCCTCTG13370.12374530864597458No Hit
GAGAAAGAGAGGGATGTGTTGCAGGAGTGGAGGGGAGAGGAGGCAGGTAT13300.12309742744887524No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT13220.12235699179504742No Hit
AGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCGGTTCTGTCTGGAGTG13170.12189421951140503No Hit
GCCAAGGAGTGGCTGCGGACACAGGACAGGAGAAGTGGCTCGATTAGATT13090.12115378385757719No Hit
CTCCAGTCCACCTCACCCCCCTTCACATGGGCCCACTTAACCCTTCCACC13070.12096867494412024No Hit
CACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGC13040.1206910115739348No Hit
ATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCT13020.12050590266047785No Hit
CTGAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGGGC12950.11985802146337853No Hit
GCTTATGCTTAGTAACCTTTTGAGGACACTGCTACCCACTAAGATGTTCT12900.11939524917973614No Hit
GGAGTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAG12870.1191175858095507No Hit
CCTCTGGTGAGCGGGCGCAACAGCATCGGGCGGTTATACTCCTCTCCTCT12850.11893247689609374No Hit
AAGCAGAACAGAATGACACGAAAGAGGAGAGGCGCAGCGGGCAGAGGAGG12810.11856225906917983No Hit
CCTTATTACGACATGGTATTAAATGGGCGCTGTGCTGCCAACATAGCAGC12770.11819204124226591No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT12600.11661861547788181No Hit
CATCAGGCACATGGAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCC12530.11597073428078244No Hit
GCTTACAGCTGCCCATTCCCACCAAACTACTGTGCACAGATTCTACTCTG12490.11560051645386855No Hit
CACACACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGT12440.11513774417022617No Hit
CATATACATTTAGTCATTTAGCAGATGCTTTTTTATATTAGGGGTAAAAA12400.11476752634331225No Hit
GTTATACTCCTCTCCTCTCTCATTATTACTCTCTCTCTCTCTCTCTTTCT12400.11476752634331225No Hit
GTTCAGACGGATGTGTGCTAGCCATTAGCCTTGCACCTCTCCGCTTGTAC12260.11347176394911355No Hit
CATGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAATCTCAAGTGCTCCCC12250.11337920949238509No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG12230.11319410057892812No Hit
AAAGAGGAGAGGCGCAGCGGGCAGAGGAGGCTGTTAATCTATCACACGCC12160.11254621938182878No Hit
CTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCAT12050.11152812035781554No Hit
CACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTGAT11940.11051002133380228No Hit
GGATTTACTGCTATCTGAAATAGAGACATTCATCAGGCACATGGAGTACC11790.10912170448287511No Hit
TTGCAGGAGTGGAGGGGAGAGAAGAGAAAGAGAGGGATGTGTTGCAGGAG11740.10865893219923273No Hit
GAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTA11630.10764083317521948No Hit
AAATTATCCTATTGTCCGTTTCCAGTGAACACTGCAGAGTGGGACGAAGG11470.10615996186756384No Hit
CACACACACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGT11470.10615996186756384No Hit
ATCCTATTGTCCGTTTCCAGTGAACACTGCAGAGTGGGACGAAGGCCATA11450.10597485295410687No Hit
AAGCAGGACAATGATCCGAAAGCACATCTCAAAATCAACACAAAAATGGT11330.10486419947336514No Hit
GTTTCGGACTGGTGGTACACACAAAACCTTACAAATGAAACAGAGAAAAT11290.10449398164645123No Hit
CAGCAGGGGCAGCGGAGAGGTGAACGGGAGGTAAGGGGGCATTTAAAATT11270.10430887273299427No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC11240.10403120936280884No Hit
GAAGAGAGGAGAGAGGGGAAGCGGGGATCATATCCCGTCTGTTCTCCTCT11160.10329077370898102No Hit
GAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGGAGAGA11150.10319821925225253No Hit
ATTTTATCCTGGAGGCCTTCTCTGGTGGAATCCGCGCACACTACACGACA11060.10236522914169625No Hit
CATTGAGGGAGGGAGTGAAAAGGAAGATTATGCTATCATATGGAGAGAAA11050.10227267468496777No Hit
GTTAAATCTCAAGTGCTCCCCTGATAGACTCTTTATTTTATCCTGGAGGC11010.10190245685805387No Hit
AGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGGAG11000.10180990240132538No Hit
ATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTAAC10970.10153223903113995No Hit
GGAGATAACGGCCCATAAAGGGCCCCATTGAGGGAGGGAGTGAAAAGGAA10960.10143968457441147No Hit
TATTACGACATGGTATTAAATGGGCGCTGTGCTGCCAACATAGCAGCTGT10830.10023647663694125No Hit
GATGTGTGCTAGCCATTAGCCTTGCACCTCTCCGCTTGTACCGTAAGGCC10810.1000513677234843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAATG100.0070863145.031753
AGCGAAA7600.0117.410141
GCGAAAG9700.091.961632
GCAGAAC2450.091.754783
TTTAGGG608.2945917E-1084.58228145
TCCAAAA353.4376146E-582.85612145
ACTGATC801.8189894E-1281.5614858
CCTATGA3200.077.055252
GCAGGAC3650.075.495983
GCCTATG3400.072.5461041
TCCAGTC3100.072.522592
CTGATCA903.6379788E-1272.499099
CTATGAG3550.069.451833
GACTGAT955.456968E-1268.686537
AGAGGCC1600.067.96798
TATGAGG4000.065.261274
GTAGCTC902.8194336E-1064.458563
AGTCCAC3500.064.225385
CGCCCAA350.00356675762.142086145
TAATGAC2200.059.328434
Produced by FastQC (version 0.11.5)