FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084846
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC31390.28934982476775506No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT31010.28584702344848945No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC21500.19818481148476375No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC20030.18463450111813107No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG19510.17984119404966234No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC16850.1553215848148032No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT16850.1553215848148032No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG16200.14932995097921734No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC15430.1422321693586002No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG14540.13402823995295185No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA13460.12407290988767068No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA12680.11688294928496763No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG12280.11319579000153017No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA12110.11162874730606925No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA11470.1057292924525693No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT10890.10038291149158497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2250.074.168051
GCGAAAG2850.058.521332
TAAGATG10900.049.893735
GCATTAA11850.044.0844731
GGGCGAT855.3379386E-542.652287
CGAAAGC4100.042.4461753
GTCTAGT6700.041.1509931
CATTAAG13650.038.781212
TTAAGAT13550.038.530484
AGATGTA13000.038.4854777
ATTAAGA13650.037.7169763
GTAGAAA11050.036.749972
GGCGATC1102.4351935E-432.957068
TAGTACT10500.031.7672564
AGACGCG1153.1625704E-431.521236145
ACCGTGC13200.031.3092088
TCCACCG13700.029.1107445
ATGGTCG200.00607589929.000874125-129
CGCCGCG200.00607726728.99954130-134
AGTAGAA14050.028.9189971
Produced by FastQC (version 0.11.5)