FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645135
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA43030.6669921799313322No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT41360.6411061250745969No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG41270.6397110682260302No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA25490.39511110077735667No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT21200.3286133909956831No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA20340.31528284777604687No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC19880.3081525572167066No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG18450.2859866539561487No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA16110.24971517589341768No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT15730.24382493586613654No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC15720.24366992954962916No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA13960.21638881784432715No Hit
CTTATGTACCATGGAGTATGTTTCTGCTCGCTTAAGTAACCTCTACATCC13580.21049857781704606No Hit
CTAGAAGCCCATCTGTGGCATTCTTAAGATTTCATTTATCTGACAGAGAA12950.20073317987707998No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA12530.19422291458376928No Hit
CTCATGGACCAAGCAACGGCACTCTGGGGAACAAACAGGACACAAAAACT11910.18461252296031064No Hit
CTCTAGGCACCTTGTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAAC11800.18290745347872925No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT11600.1798073271485813No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG11600.1798073271485813No Hit
CATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTG11520.1785672766165221No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA11340.17577716291938897No Hit
GTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTTAAGCGGGAAG11240.174227099754315No Hit
GTTCTGGTTTTGCATGTAATTTTGTTTCCATGCACATGTCTGTATCAGCA11150.1728320429057484No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT10760.1667867965619599No Hit
GTTCTAGAGATTGGATACACTCACAAGAGGCAATGTAAGCTTTCCGTAGA10700.16585675866291552No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA10600.16430669549784155No Hit
GTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCATTCTTAAGAT10420.16151658180070838No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT10050.15578134808993468No Hit
CCCATATTTCAACTCTTAAGCGGGAAGAAAATGTTGACACCTGCTGTACC10010.15516132282390507No Hit
TCCGTAGAGTGTCTGAGCCTCTGACAAGAGTTGTAAAACCAGACATGCTC9770.15144117122772754No Hit
TGTCTGTATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTTCTCGG9560.14818603858107215No Hit
GCATTATTTTGACCCATAGTGCTGCTGCTGTTGGATGTATAGCCCAGTCC9420.1460159501499686No Hit
GTGTAAGTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACC9080.1407457353887171No Hit
GTCATTAAGTGGCTGCCTTCAGCTCAGGTCATGATCCCAGGGTTCTGGGA9080.1407457353887171No Hit
GTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTGAGCATCT9060.1404357227557023No Hit
CCTTTATTGCCCGGGCTCAGGTCTGGCCCCTGGGTCCTGCTCCCTCTCCT8710.13501050167794337No Hit
ATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTTAAGCG8670.1343904764119138No Hit
GGTCTGGCCCCTGGGTCCTGCTCCCTCTCCTGCCCTCTAGGCACCTGCCT8270.12819022375161787No Hit
GTTAATATACAGTGCGATATTGGTTTCAGGAGTAGAAATCAGTGATTATT8140.1261751416370217No Hit
GTATATGGGAGATCACAAACATTACATACAGATGCATGCACACACATGTG8130.12602013532051431No Hit
GTCTCTATGAGCTTGAGGACTCTAGGCACCTTGTGTTAAGTAGAATCACA8050.12478008478845513No Hit
ATTTTGACCCATAGTGCTGCTGCTGTTGGATGTATAGCCCAGTCCTAAGT7960.12338502793988854No Hit
GGACTAAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAG7890.12229998372433677No Hit
GTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAACTGGCATATCTTAC7790.12074992055926279No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC7760.12028490160974059No Hit
GAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGAGT7700.1193548637106962No Hit
TTCTATTTCTCTGTCTCTATGAGCTTGAGGACTCTAGGCACCTTGTGTTA7660.11873483844466663No Hit
GACTAAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGA7510.11640974369705566No Hit
AAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGATGAG7500.11625473738054826No Hit
GTTCTGGTCTGTTCTGGTTTTGCATGTAATTTTGTTTCCATGCACATGTC7500.11625473738054826No Hit
GTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGATGAGAAAGTTCTAGA7380.11439466158245949No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT7350.1139296426329373No Hit
GAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGA7280.1128445984173855No Hit
GCTGTATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTT7160.11098452261929673No Hit
CCTCTACATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCT7150.11082951630278932No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC7100.11005448472025237No Hit
GTACCACAGGTGAGCCTTGAGGGTATTCCGCCAAGTAAGATATGCCAGTT7070.10958946577073016No Hit
GTATGTTTCTGCTCGCTTAAGTAACCTCTACATCCAACACGGGGCTCCAA6960.10788439628914878No Hit
ATCAAGAGCTGTATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTC6820.10571430785804523No Hit
CTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCATT6820.10571430785804523No Hit
TCCTTACATTGTGGAAAGGGAGCCCAAAGGGGAATGGATGTGCTTGGGGA6670.10338921311043425No Hit
GTTCACAAATACATTGAAAGTGGCTTTTTCTCGGTTTATGCTCTCACAGG6650.10307920047741945No Hit
CCCCATGACCACCTCCCTTTAGTCCCTGGTAACCATCATTCTATTTCTCT6600.10230416889488246No Hit
ATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCTGTATGCT6520.1010641183628233No Hit
GCTCTGAGCCTTTTATGTAACAACAGTCAGTGTGCAAATCAGTGTTCTGG6520.1010641183628233No Hit
TATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTTCTCGGTTTATG6490.1005990994133011No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA100.007088799145.010155
TCGGACT100.007088799145.010155
GCTCGAA100.007088799145.010158
AGCGAAA2100.096.7109151
GCGAAAG2500.081.211982
CGAAAGC3050.066.5620353
TCGTTGG451.19358236E-464.443969
GTCTATG1700.063.9800262
CAGTCGT350.00356494162.1472056
ACTCGGT502.0090117E-458.0040638
CTATGTA1750.053.8609164
TATAGAC553.2158004E-452.7350542
TAGGTCG1258.913048E-1152.2036555
GATTATA604.940172E-448.3404662
CCACTAC604.942061E-448.336723
CCCCGTA450.00963788148.3329739
GAGTTCG752.5496816E-548.332979
CAGCTCG17300.044.8407069
ATTAGGA657.3293096E-444.6219672
GTATATG3100.044.4558221
Produced by FastQC (version 0.11.5)