FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066178
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC19720.18495973467844956No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT11700.10973777361753853No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT10840.10167157829180493No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA10820.10148399235399717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.078.574181
GCGAAAG2850.066.1553042
CGAAAGC3550.053.1056183
GTAGAAA7650.042.6647071
ATCCGTT800.002036759436.2496957
GTCTAGT3050.033.292461
TCGCGTT1102.4363775E-432.95427145
GCGGTAA1255.1543186E-428.999756145
ACCGTGC3600.028.1942088
GGATTAG4900.028.123881
AGTACTG6050.026.3671235
TAGTACT6000.025.3783594
CCCTATA2101.5502183E-524.1766681
GAGCTAT3601.8371793E-1024.1698656
AGACCGC1500.001494410724.166464145
AGTAGAA11250.023.8543131
CGTACAC1550.001807175623.3901923
GCATTAA4950.021.978791
TTAGCAC4307.094059E-1121.9215053
TTTATAC4307.094059E-1121.9215053
Produced by FastQC (version 0.11.5)