FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055511
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC22340.21165103916491634No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA16900.16011202157059473No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT13060.12373153856283829No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA12790.12117353585135542No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA12580.11918397818686872No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG11990.11359426855807282No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT11460.1085730039762731No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT10950.10374122107680546No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC10630.1007095141594924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.074.024411
CGAAAGC3400.053.318373
GCGAAAG3500.051.797442
TAGGCGC450.00963426948.33975
GCATTAA6600.040.668561
ATGAACG2350.040.1116646
TACCGTG1001.3911992E-436.251347
TGTACCG1051.8523517E-434.5283555
AACCGTA1051.8533836E-434.5250857
TAGTACT7400.034.2950554
GAAACCG2558.913048E-1131.2786276
TGAACGT3250.031.2319247
GAACGTC3350.030.2981918
GTCTAGT3600.030.2266331
GGACAGT10000.029.003826
GACAGTG10100.028.713937
TAGCACA4100.028.2964084
TAAACCG1555.4957753E-528.068215
TACGCCC1306.4782007E-427.8882884
ACCGTGC4450.027.696348
Produced by FastQC (version 0.11.5)