FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619232
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT166610.6361024911118984No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA94150.35945651244334215No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG79420.30321865340680015No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA71930.27462248475889117No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT71720.2738207230210993No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC58850.22468418223357076No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA54480.20799990226142623No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG54450.20788536487031314No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT54360.20754175269697378No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA54200.20693088661103712No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT50510.19284278750412334No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG49800.1901320692477795No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT46740.17844925535424125No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT46150.1761966866623499No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC45990.17558582057641325No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC44580.1702025631940966No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG44190.168713577109626No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT39030.14901314583816935No Hit
CCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAGCAGAGACAGAGG37930.14481344149735495No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT36760.14034648324394325No Hit
TTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGAT36520.1394301841150383No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG36430.13908657194169893No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC36240.13836116846464916No Hit
CTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAG34770.13274883630010628No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC34490.13167982064971717No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA34270.13083987978155429No Hit
CCCTCAGCCACCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACAT33790.12900728152374438No Hit
CATCTACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTT33360.12736557891778966No Hit
GTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGAT33140.12652563804962677No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG33110.12641110065851363No Hit
GCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAAC32870.12549480152960868No Hit
ATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCCCTAATG32730.12496029370441411No Hit
TCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAA32470.12396763631476707No Hit
GTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCTGGAAGCCAGAACCAAG31860.12163870936213364No Hit
CATAAAGTGCTTTGTACATGTCTGGCACCTGATAAGAGCTGATAACTGTA31580.1205696937117445No Hit
GTCCAAATCCCTCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGG31370.11976793197395266No Hit
GTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCC31060.11858437893245043No Hit
GTGCAAGGGACTGCAGGGGGTTTGTGGTCCTCAGAGCTTTGCGGGGGAAG31010.11839348328059522No Hit
CTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTCATT31000.11835530415022419No Hit
CCTATTGCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTA30760.11743900502131924No Hit
TTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAACAGGTA30320.11575912328499345No Hit
GATAAGAGCTGATAACTGTAGATGGGTTTTGTTTAATTTTTTAAAAGCCT30030.1146519285042333No Hit
GCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTAAACAAA29470.11251389720345506No Hit
GGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAACAGGCCCCCAG29460.11247571807308401No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA28720.10965046242562705No Hit
CCTAGTGCCTGGCACAGGGTGGGTCCTCCCTAAATGTAAACCTCCCTCCC28680.1094977459041429No Hit
CGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGGCA28210.10770332677670401No Hit
GTCTCAGTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCT28110.10732153547299361No Hit
CCCCCTGCAGTCCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCG27670.10564165373666784No Hit
GCTCTGAGGACCACAAACCCCCTGCAGTCCCTTGCACTTTAGAATGCAGG27660.10560347460629681No Hit
GGTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGC27550.10518350417221536No Hit
CCCTTTACAGGAAATGTGACAAAAATCTTCAGAATTGCTGAAATCGCTGA27200.10384723460922897No Hit
CCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTTC27120.10354180156626064No Hit
TATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTG26800.10232006939438737No Hit
GGTTTACATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAA26430.10090744157065887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6900.073.584761
GACAGTG54700.055.1426247
GCGAAAG9500.053.4365962
GGACAGT60550.051.8576976
CAAGGAC56850.051.0243233
CGAAAGC10100.050.2602233
TACTACG450.00963124848.3455473
ACAAGGA61200.048.2288932
AACAAGG64050.047.33651
CAGTGGG65900.044.9955529
AAGGACA70700.041.4382484
ACAGTGG81700.038.958958
GTAGAAA20150.038.8765451
AGGACAG78150.038.694265
ATATACG1001.3899968E-436.2591633
AGTAGAA18750.032.8818781
TAGAAAC24400.030.613412
GGGCTGA35950.029.659061
ATCGCTG93000.028.687754145
TCCGGAT1855.80943E-627.4393623
Produced by FastQC (version 0.11.5)