FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492411
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCCA24000.487397722634141No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT20230.41083566370369473No Hit
GTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGT18990.38565344803426405No Hit
AAGCAGGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTC17520.35580033752292295No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG16870.34259998253491497No Hit
GTGCATGTGTGCATCACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACC16170.3283842156247525No Hit
TATTATAACACTGGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAG16010.3251348974738582No Hit
GTTTAGGACAGTTCATTTTCTTACATATGGTTGTTTACTTGTTCTAATAT15720.31924550832536236No Hit
TTATAACACTGGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAGAA15560.31599619017446806No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC15520.31518386063674453No Hit
TCTTTGGGCTGATGGGGAAGCGGGTGAGAGGAATACCTCCCATCCGGCCG15080.3062482357217853No Hit
GGTGAAGAGGGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCGGG13930.28289376151223267No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC13890.28208143197450913No Hit
GTCCTAAACCTCATACCTTCCATAAAAATTACTCAAATGAGATGACAAGA13690.27801978428589125No Hit
GCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAA13530.27477046613499695No Hit
CAGGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCG12360.2510098271565826No Hit
TTGCTGAACCTATCGCACGGGCCCCTGTCTGCACACCTGCTGTCATTCAC12230.24836975615898102No Hit
GGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATG12160.24694817946796477No Hit
GTGCATCACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAG11380.23110775348235518No Hit
CCACCAGAGCATCCGCAAGGACAAATGGTGCAGGGCCTTGTGGACAGGGC11050.22440603479613577No Hit
AAGCAGGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCA11000.22339062287398131No Hit
ATCTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCCC10850.22034438710751791No Hit
GTGCAGGGCCTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGA10750.218313563263209No Hit
AAAGCAGGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCAT10670.21668890418776185No Hit
TCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTG10640.21607965703446916No Hit
ATTTATAATAGCTCTAACAAAAGGAGACTTACCACAGGTCACGGACTAAA10380.21079951503926597No Hit
CAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACCGAGAG10280.20876869119495706No Hit
GTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCCAGGTGGGACACACCA10250.20815944404166437No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA10070.20450396112190833No Hit
GGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCAGAAACTCACTGA9790.19881765435784335No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC9770.19841148958898158No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA9690.19678683051353443No Hit
GGTGAGAGGAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCAC9570.19434984190036372No Hit
CACCAGTGTCAGGCACAGGAGACACACGAGAAGAGAGTTGGTCTGGCCAG9520.19333442997820927No Hit
ATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGGAG9470.1923190180560548No Hit
TCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAG9450.191912853287193No Hit
GAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTT9100.1848049698321118No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT8940.1815556516812175No Hit
GGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAGAACATAGTCCAG8930.18135256929678664No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC8920.18114948691235572No Hit
TAGATAAACTGAGTCTAAAATTCATATTGAAAGGCAAAAGAACTAGGAAA8910.18094640452792485No Hit
GGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCAGAAAC8850.1797279102213395No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA8780.17830633353032324No Hit
GACAAATGGTGCAGGGCCTTGTGGACAGGGCGCAACTATCTATAGAAGGC8480.17221386199739647No Hit
GCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGA8470.1720107796129656No Hit
ATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCCCCACT8430.171198450075242No Hit
GTCCCAGGACTCTGGAATGATTACTGGAGCTGAAGGCAGACACTTAACTG8320.16896454384650222No Hit
GAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAGCAT8160.16571522569560793No Hit
AGCGAAAGCAGGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCAT8060.163684401851299No Hit
CAGGTGAAGAGGGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCG7940.1612474132381283No Hit
GGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCAT7940.1612474132381283No Hit
GTCAAGATCTATCTAGTCTGAGTTTCTAAGCCAGGTCTGTGTGTTTATGC7830.1590135070093885No Hit
GACATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGCTT7730.15698268316507957No Hit
GGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGC7700.1563734360117869No Hit
GTATGAGGTTTAGGACAGTTCATTTTCTTACATATGGTTGTTTACTTGTT7680.15596727124292512No Hit
TTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGGCT7600.15434261216747797No Hit
GATGGGGAAGCGGGTGAGAGGAATACCTCCCATCCGGCCGGACAGAAGAA7580.1539364473986162No Hit
CCTTTGTACAGCAAAAGTTTTTAATTTTAATGAAGTCCCATTTTTAAGTT7570.1537333650141853No Hit
CTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTA7530.15292103547646174No Hit
GACTTGGTGTTCTTCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAG7520.15271795309203084No Hit
GACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGTGACATAAGATGTGA7480.15190562355430728No Hit
TATCTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCC7280.14784397586568943No Hit
GATAGGAGCAGAGGGAGAAAAAGAAGACTCTCTTCTGAGCAGGGAGGCTG7230.14682856394353497No Hit
TTCTTGGCCACCTCATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT7220.14662548155910407No Hit
GTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGAT7130.14479774009922605No Hit
CCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAGCATCCGCA7120.14459465771479516No Hit
CTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGATGAGGACCA7090.1439854105615025No Hit
GAGGAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAG7000.14215766910162445No Hit
CAGTTACAGGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGC6990.14195458671719358No Hit
GTTCAGGGAGGCCAGGTGGGACACACCAGTGTCAGGCACAGGAGACACAC6900.14012684525731553No Hit
CTTCTGAGCAGGGAGGCTGTTGCGGGGCTCAGTCCCAGGACTCTGGAATG6830.13870526856629928No Hit
GAGTTCAGGGAGGCCAGGTGGGACACACCAGTGTCAGGCACAGGAGACAC6790.13789293902857572No Hit
GTCCAGAAATAGACCCCACAAAATTTGGCAAATTAATTTTTGACAAAGGT6720.13647136233755947No Hit
AGCAGGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCA6680.1356590327998359No Hit
CCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAACCTATCG6640.13484670326211234No Hit
GTTTTACATTTAGTCTTGTCATCTCATTTGAGTAATTTTTATGGAAGGTA6640.13484670326211234No Hit
GAAGAACAGCTCCACCACCAGAGCATCCGCAAGGACAAATGGTGCAGGGC6620.13444053849325055No Hit
GAAAGCAGGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGT6550.13301896180223433No Hit
GATGAGGACCACGGGTCTGAGTGAGCACCCCCACTGCACCCTTCCTGGAG6520.13240971464894163No Hit
GCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACC6510.13220663226451074No Hit
GCAGGAAGGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGC6460.13119122034235628No Hit
ATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGA6460.13119122034235628No Hit
GTGACATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGC6350.12895731411361647No Hit
GGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCC6140.12469258404056774No Hit
CATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGG6140.12469258404056774No Hit
GTCTGCACACCTGCTGTCATTCACCATCAGCTTCAGCATTTAAACCCTGT6110.12408333688727508No Hit
CTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGTGACATAAG5950.12083401873638079No Hit
GGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGAT5910.12002168919865722No Hit
CATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGCTTCC5910.12002168919865722No Hit
GTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACT5870.11920935966093366No Hit
ACCCTGAACTGTGTGAACCCCGAGAACGAGAACGCACCTGAGGTGCCAGT5860.11900627727650274No Hit
GGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCGGGTGAGAGGAA5770.11717853581662474No Hit
GAAGGAGGAGGTGCGGCTACAGTATGAAAAGCTGCACCAGCTGCTGGACG5620.11413230005016135No Hit
CTTCCAGATAATCTAACTTGACGTAAACCTTTGAACTTGAAACCATACTT5600.11372613528129957No Hit
GGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATC5600.11372613528129957No Hit
GATCTATCTAGTCTGAGTTTCTAAGCCAGGTCTGTGTGTTTATGCCAAGT5560.112913805743576No Hit
GCCTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGATGAGGAC5530.11230455859028332No Hit
GATAAACTGAGTCTAAAATTCATATTGAAAGGCAAAAGAACTAGGAAAAT5520.11210147620585242No Hit
GTTCTTGGCTGACTGACAGATTGCTGAACCTATCGCACGGGCCCCTGTCT5520.11210147620585242No Hit
CACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGG5490.11149222905255975No Hit
CCCTTGTACACGGCATCCAGCAGCTTCTCCTTGACCTGAGTTACCGTGTC5480.11128914666812886No Hit
ACTATCTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCAC5480.11128914666812886No Hit
GGCCCCTGTCTGCACACCTGCTGTCATTCACCATCAGCTTCAGCATTTAA5470.11108606428369795No Hit
GTGTCAGGCACAGGAGACACACGAGAAGAGAGTTGGTCTGGCCAGCTGTC5440.11047681713040529No Hit
GACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCG5350.10864907567052727No Hit
CCTTTATTCTCTTCTAAAAGTTTTACAGTTTTACATTTAGTCTTGTCATC5350.10864907567052727No Hit
GTGGAGGTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC5340.10844599328609637No Hit
ACATAATTGTACTCACGCTAATGAGACGTTGCACAGCGAGAGGGGTCCAC5310.1078367461328037No Hit
GTTATAATATGAACATTTTCTTGGTTATGTCTTAAATTTAGATACTGTGA5300.1076336637483728No Hit
TTTTAACAGTGTCCTTTGTACAGCAAAAGTTTTTAATTTTAATGAAGTCC5260.10682133421064924No Hit
GAAATAGACCCCACAAAATTTGGCAAATTAATTTTTGACAAAGGTGCAAA5250.10661825182621833No Hit
TGTCTGCACACCTGCTGTCATTCACCATCAGCTTCAGCATTTAAACCCTG5160.10479051036634032No Hit
CAGAAGAACAGCTCCACCACCAGAGCATCCGCAAGGACAAATGGTGCAGG5120.10397818082861675No Hit
GCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAACCTATCGCACGGGC5050.1025566041376005No Hit
GGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCT5050.1025566041376005No Hit
CTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCCCCA5050.1025566041376005No Hit
GAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACCGAGAGTTCTAG5040.10235352175316961No Hit
ATCTAAATTTAAGACATAACCAAGAAAATGTTCATATTATAACACTGGCA5040.10235352175316961No Hit
GCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCA5010.10174427459987694No Hit
GTTCTAATATGATTTGCTGGGAAGACTCTCCTTTCTCTTTTGAATTGTGT5000.10154119221544604No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC4980.10113502744658424No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA100.0070900745144.99797145
AGCGAAA4150.0134.598191
GAGCGAA406.091195E-790.678981
GCGAAAG6250.090.50632
GAGGTCG755.638867E-1177.347966
ACAACGT300.001930590772.543181
TTGTCCC406.6602945E-572.498985145
GATTCTA406.6602945E-572.498985145
GCTGTCG1050.069.0536658
CTGTCGG1050.069.046659
GGTCGTC851.6916601E-1068.2412648
GTTCTTA451.1933417E-464.44354145
TCCCCGC350.00356562762.141983145
AGGTCGT1007.166818E-1058.0050777
TGTCCGA651.091601E-555.7797786
GTCCGAC651.092258E-555.7741137
TATGTTG651.0929154E-555.76845145
AGAGGTC1051.1059456E-955.2485435
GATAGGA2500.055.132821
ATAATTG2150.050.59613
Produced by FastQC (version 0.11.5)