FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046643
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA39030.19070253092503187No Hit
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT38780.18948101842871473No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC35970.17575121797011006No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA21510.1050989351831267No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC21400.10456146968474717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7200.048.352911
GTAGAAA20700.046.600991
CGAAAGC8400.041.434213
GCGAAAG9400.037.0290342
TAGAAAC26050.035.909632
GGACAGT30900.034.258656
ACCATCG9700.032.887068
GACAGTG31200.032.7664837
TGAACGT6950.029.2104687
CAATTCG3001.8189894E-1128.9996999
AGTAGAA20300.028.9402891
GAACGTC7200.028.194848
CTCCGAC1555.50267E-528.064909145
GCAGGAC31800.025.0820313
ACAAGGG21050.024.455227
AGTGATC24350.024.4151278
GAAACAA38350.024.2002894
CATACCG1500.001494897124.1664169
GTGATCA24100.024.0661379
AACGTCA9200.022.8530229
Produced by FastQC (version 0.11.5)