FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833587
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATATATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATT168092.0164661876924663No Hit
ACTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAA78630.9432728677390602No Hit
TTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTG67580.8107132188961681No Hit
GGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTC47540.5703063987322259No Hit
ATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATGCACT44670.5358768790780086No Hit
ATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAGG44620.53527706166243No Hit
GGTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCC41890.5025270307718331No Hit
GATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAAT41710.5003676880757497No Hit
TTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGG41400.49664882009916184No Hit
GTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGT38630.46341893527610195No Hit
GTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTCAC36230.43462769932832446No Hit
GAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAA35210.422391424050519No Hit
ACCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAG35170.421911570118056No Hit
CATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAAAAAAAAAA34250.41087492967140804No Hit
GAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGA34180.4100351852895978No Hit
GTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTG33120.39731905607932944No Hit
GTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCT32670.3919206993391212No Hit
GTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTT29630.35545180047193636No Hit
TATCAGGGCCTCTCGCAGAAGCCACAACCCCCAACCACACAACTTTATGA29440.3531724942927373No Hit
TTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCA28520.34213585384608924No Hit
CCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTC27800.333498483061756No Hit
GTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGGAAGAAAAAACA27790.33337851957864023No Hit
CTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAAA27400.3286999437371264No Hit
TTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTAT27060.3246211853111913No Hit
CTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGAGAACTGTTTGA25320.30374753924905257No Hit
TATATAAACTGAGCACTCAGGCATCACACACAAACAGTACACAAATGAGA25270.3031477218334739No Hit
GAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATC24550.29451035104914064No Hit
GAAAGAATACTGTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGG24180.2900717021738583No Hit
AGCAGGGAGTGAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGT22630.2714773622909187No Hit
CATTTGCACGTTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTT19840.23800755050162728No Hit
AAAGCAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGG19400.23272915724453477No Hit
ATCTCACACTCTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTT19360.2322493033120718No Hit
TAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCA19230.2306897780315672No Hit
CATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGC18830.2258912387069376No Hit
GAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATC18490.22181248028100248No Hit
TCTCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATG18350.2201329915173821No Hit
GACATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAAT17880.21449470781094235No Hit
CCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCG17670.2119754746655118No Hit
ACATGAAGAAGGCGATCTCTCAACACATTTATCTTCTTTTTTTTTCCAGT17640.2116155842161646No Hit
GAATAGAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGAAGGG17600.2111357302837016No Hit
GAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATCATTTGCACGTTA17550.21053591286812295No Hit
GTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCCA17280.20729689882399796No Hit
CTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGA17230.20669708140841928No Hit
CTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTCTCTCTCCTTCCATT16970.20357803084741005No Hit
ATATATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATG16380.19650018534358143No Hit
GGTGAAGGAGATGAAAAAAAAAAACAGAAAGAATACTGTTTAAAGATCAA16350.19614029489423418No Hit
GAAGAAGGCGATCTCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGG16240.19482069657996104No Hit
GTATTCTTTCTGTTTTTTTTTTTCATCTCCTTCACCTAACTGGAAAAAAA16070.19278131736699347No Hit
GTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATGCACTCT15980.19170164601895184No Hit
ATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCT15980.19170164601895184No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15880.19050201118779442No Hit
TCTTTAAACAGTATTCTTTCTGTTTTTTTTTTTCATCTCCTTCACCTAAC15740.18882252242417408No Hit
GCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGA15620.18738296062678522No Hit
GTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAATGAAAGAAAGAGA15610.18726299714366948No Hit
GCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCTT15570.1867831432112065No Hit
CTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTT15530.18630328927874354No Hit
CTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTC14890.1786256263593362No Hit
GACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCTTCTTTT14550.17454686793340107No Hit
CCATTTATCAGGGCCTCTCGCAGAAGCCACAACCCCCAACCACACAACTT14340.17202763478797053No Hit
CTCTTTCACTCCTTCTCTCTCTCCTTCCATTCTATTCCCTCTCTCCCTCT13960.16746902242957243No Hit
TCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATA13830.16590949714906783No Hit
CCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGAT13550.16255051962182712No Hit
ATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTCACGTGGGAGCTCA13050.15655234546604013No Hit
GAATACTGTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGGAAGA12990.1558325645673457No Hit
TCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAA12970.15559263760111422No Hit
CTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATC12930.15511278366865125No Hit
GGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAG12920.15499282018553553No Hit
CATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAATGA12880.15451296625307256No Hit
GTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGTGT12880.15451296625307256No Hit
CGTTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGG12770.15319336793879942No Hit
TCATAAAGTTGTGTGGTTGGGGGTTGTGGCTTCTGCGAGAGGCCCTGATA12750.15295344097256797No Hit
GTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTCTCTCTC12740.15283347748945222No Hit
CTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTT12700.15235362355698925No Hit
ATTCTATTCCCTCTCTCCCTCTTTCTATAGCTACCCTCTCCCTCTCTCTC12580.15091406175960037No Hit
ATATAAAATAGGGTGATATGTGAATTGAATGAAACCCAAAGACTGTATGC12380.1485147920972856No Hit
ACCCTGGGTTGTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGT12320.14779501119859115No Hit
GAGAAGGACATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAA12290.14743512074924392No Hit
TTCTATAGCTACCCTCTCCCTCTCTCTCTCTCTCCTCTCACGCTCTCTCT12260.14707523029989672No Hit
GAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAG12210.14647541288431803No Hit
TCCTCACACTGGTCATCAGACACAGGCTGCATGGTGGACAAGAGCTTTGA12050.14455599715446618No Hit
TTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTC12020.14419610670511895No Hit
ATTTTATATGGTCCTTCGAGCCGGATATTACATTTACCGCAGTTAAAGAT11880.1425166179414986No Hit
AGCAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCG11670.13999738479606808No Hit
CAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCCACAAC11600.1391576404142579No Hit
GTTGAATAGAATGAAAGAAAGAGAGAGAGCGTGAGAGGAGAGAGAGAGAG11590.13903767693114216No Hit
GAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATCATTT11590.13903767693114216No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT11580.13891771344802642No Hit
GGACATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAA11510.13807796906621625No Hit
GAGTAGCTATAGCAAGAGGGAGAGAGGGAATAGAATGGAAGGAGAGAGAG11360.13627851681948014No Hit
AGATATGCGTGTCCTGCCAGAGTTGAATAGAATGAAAGAAAGAGAGAGAG11310.13567869940390145No Hit
GCTCATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATA11300.1355587359207857No Hit
AATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTG11300.1355587359207857No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT11290.13543877243766997No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG11260.13507888198832277No Hit
GAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGAAGGGGTAGA11240.13483895502209128No Hit
AGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCT11110.13327942974158666No Hit
CAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTC10850.13016037918057743No Hit
GAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATC10790.129440598281883No Hit
CCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAGA10740.1288407808663043No Hit
CCTTCACCTAACTGGAAAAAAAAAGAAGATAAATGTGTTGAGAGATCGCC10430.12512191288971639No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC10390.1246420589572534No Hit
AAAGCAGGGAGTGAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTT10250.12296257019363305No Hit
TCCATACATATATAAACTGAGCACTCAGGCATCACACACAAACAGTACAC10240.1228426067105173No Hit
AAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAG10180.12212282581182288No Hit
CTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAAC10160.1218828988455914No Hit
TCCTTCACCTAACTGGAAAAAAAAAGAAGATAAATGTGTTGAGAGATCGC10140.12164297187935993No Hit
GTTGTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGC10110.1212830814300127No Hit
TTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAG9860.11828399435211921No Hit
GAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGTGTATATCTCATTTGTG9600.11516494379110997No Hit
TATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAG9490.11384534547683686No Hit
GCCCATCAGGCAGAGAACTGTTTGATCTTTAAACAGTATTCTTTCTGTTT9400.11276567412879519No Hit
AGCCACAACCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAA9370.11240578367944798No Hit
GAGGGAATAGAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGA9370.11240578367944798No Hit
GTGAAAGAGAGAGGGAGAAGGGGTAGAGAGAAGGACATAGGGAGAGTGTG9350.1121658567132165No Hit
GATAGGGAGAGAGGGAATAGAATGAAAGAAAGAGAGAGAGAGCGTGAGAG9350.1121658567132165No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA9320.11180596626386928No Hit
GAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGT9140.10964662356778597No Hit
GGCAGAGAACTGTTTGATCTTTAAACAGTATTCTTTCTGTTTTTTTTTTT9050.10856695221974431No Hit
AAAGAATACTGTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGGA9050.10856695221974431No Hit
CTCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGA8970.10760724435481839No Hit
GGATGTGAATGTCCTGTCAGCCAGTGGCGAGATAGGGAGAGAGGGAATAG8910.10688746345612396No Hit
GAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGAAGGGGTAGAGAGAA8900.10676749997300822No Hit
CTCTCTTTCTTTCATTCTATTCAACTCTGGCAGGACACGCATATCTCACA8890.10664753648989247No Hit
TGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGG8870.106407609523661No Hit
ATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTATAGCAA8760.10508801120938786No Hit
CTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGAGAACTGTTT8750.10496804772627212No Hit
TTCTTCCTCTGCCCATCAGGCAGAGAACTGTTTGATCTTTAAACAGTATT8750.10496804772627212No Hit
AGCGAAAGCAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGA8730.10472812076004065No Hit
CTATTCCCTCTCTCCCTCTTTCTATAGCTACCCTCTCCCTCTCTCTCTCT8680.10412830334446195No Hit
GTGCATGAATTAGCATCAATAATGAGCTCCCACGTGAGCATCCATACATA8680.10412830334446195No Hit
GAATGAAAGAAAGAGAGAGAGCGTGAGAGGAGAGAGAGAGAGAGGGAGAG8670.10400833986134621No Hit
TAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAATGAAA8630.10352848592888324No Hit
CTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTC8550.10256877806395734No Hit
GCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTC8550.10256877806395734No Hit
CGCATATCTCACACTCTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCT8540.1024488145808416No Hit
CGTGGGAGCTCATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATT8540.1024488145808416No Hit
GCTATAGCAAGAGGGAGAGAGGGAATAGAATGGAAGGAGAGAGAGAAGGA8480.10172903368214714No Hit
GTTCTCTGCCTGATGGGCAGAGGAAGAAAAAACAACATCACAGAGAGCAA8450.10136914323279993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5050.0116.342291
AGTATTA1950.0100.384026145
GCGAAAG7200.081.5718152
TATTCTA651.5643309E-978.07647145
CGAAAGC7800.075.292543
CAGAAAT1450.059.999657145
AGGCCCG502.0101067E-457.99966145
GCAGAGC1650.057.124313
GAAAGCA10350.056.7422074
ACTTGAG400.006039192454.4007951
TTAGACT805.8703154E-754.377954
CTTCCTA400.00605067254.374687145
GAATAGG1405.456968E-1251.8102761
GTGCCCG1750.049.7139979
GTTAGAC752.5493979E-548.3359533
AGGTGCC1800.048.3330547
TCATTAG1850.047.0323942
GGTGCCC1900.045.789218
CAGGTGT657.3339185E-444.6178056
AGCTGCT1151.4122634E-744.130187
Produced by FastQC (version 0.11.5)