FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693740
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA105511.5208867875572982No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG56010.807362989016058No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG55450.7992908005881165No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT53210.7670020468763514No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50800.7322628073918183No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT48710.7021362470089659No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA48210.6949289359125898No Hit
ATGCTAGAAATTCCAACCGGTCTCCTAGATTGCAGCACTTCTGGTACATC41290.5951797503387436No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40130.5784587885951509No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG36700.5290166344740105No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG35390.5101334794015049No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG33770.4867817914492461No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC32300.46559229682590025No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC31570.455069622625191No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG31200.4497362124138727No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG29350.4230691613572808No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG27020.3894830916481679No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC26870.38732089831925504No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT25600.3690143281344596No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT24810.3576267766021852No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT23130.33341021131836135No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT22500.3243289993369274No Hit
GAATGAAGTGGATCGACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGA20990.30256291982587136No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20080.2894456136304667No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT20070.28930146740853924No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG19890.28670683541384384No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC18750.27027416611410615No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC18240.2629227087958025No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA18160.26176953902038225No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA18060.26032807680110703No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA18010.2596073456914694No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG17460.25167930348545564No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA17010.24519272349871707No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA16970.24461613861100703No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA16700.24072419061896386No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC16610.23942687462161616No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT16120.23236370974716752No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA15010.21636347911321244No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA14830.21376884711851704No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC14770.2129039697869519No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCAC14430.2080029982414161No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA14070.20281373425202523No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA13880.20007495603540232No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT13600.19603886182143165No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA13530.195029838267939No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG13430.19358837604866377No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC13410.19330008360480871No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT13230.1907054516101133No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT12980.1871017960619252No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG12790.18436301784530226No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTAGATTGCAGCACTT12290.1771557067489261No Hit
GGATACACCATGGACACAGTACACATTCCTGGATTCCCAAGAGGAATCGT12280.1770115605269986No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA12220.17614668319543345No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT12210.17600253697350593No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA12180.17557009830772335No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA12160.1752818058638683No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT12020.17326375875688296No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG11910.1716781503156802No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11770.1696601032086949No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC11670.16821864098941966No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG11660.16807449476749214No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT11650.16793034854556463No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT11480.16547986277279672No Hit
CAAGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCAT11480.16547986277279672No Hit
CTCCTAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCC11480.16547986277279672No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC11450.16504742410701415No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC11350.16360596188773893No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT11310.1630293770000288No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11290.16274108455617378No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC10850.15639865079136278No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC10800.15567791968172515No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC10760.15510133479401503No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATCGAC10480.1510652405800444No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG10310.1486147548072765No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT10190.14688500014414624No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA10180.1467408539222187No Hit
CAGTACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAA10180.1467408539222187No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10020.14443451437137833No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG9780.14097500504511778No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC9690.13967768904777006No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC9660.13924525038198748No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTCAGCGCAAAATGC9660.13924525038198748No Hit
GGATAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGG9500.1369389108311471No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG9420.13578574105572694No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC9390.13535330238994436No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGC9350.13477671750223427No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT9210.13275867039524894No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT9180.1323262317294664No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC8970.1292991610689884No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT8940.12886672240320582No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC8800.1268486752962205No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG8770.1264162366304379No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG8540.1231008735261049No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA8510.1226684348603223No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC8450.12180355752875716No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC8200.1181999019805691No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT8170.11776746331478652No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT7970.11488453887623605No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA7970.11488453887623605No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA7960.11474039265430852No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAGTAAACAATGCTGT7960.11474039265430852No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA7760.11185746821575807No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG7490.10796552022371494No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA7420.10695649667022226No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC7230.10421771845359933No Hit
GTCTCCTAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTC7170.1033528411220342No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC7160.10320869490010667No Hit
GGATACACCATGGACACACATTCCTGGATTCCCAAGAGGAATCGTTCTAT7150.10306454867817914No Hit
TTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGG7000.1009023553492663No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG7000.1009023553492663No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT6990.10075820912733877No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG6970.10046991668348373No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT6950.10018162423962869No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA100.00709065144.99826145
AGCGAAA2050.095.5555341
AGTAGAA12000.088.8754961
GCGAAAG2600.075.309272
GCAGGTA1750.074.592052
CGAAAGC2700.072.509593
GTAGAAA15550.068.555932
CAGGCAA3850.064.034445
ACAAGGC12050.062.576388
TAGAAAC17550.061.9740073
CAGGTAC2150.060.70573
AGACGCG606.803886E-660.415943145
AGAAACA19400.054.9428334
GGATACG400.00603315854.413571
GGCAAAC4600.053.590187
CAAATAT11950.053.396184
CAAGGCA14400.052.3604859
GTACTGA2650.051.9880076
AGGCAAA4800.051.3609546
ACTGATC2400.051.3572548
Produced by FastQC (version 0.11.5)