FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228892
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC451.2330838E-680.555248
GATCGCG702.3350367E-762.1426167
AGCGAAA4450.053.7813381
TCGCGCC951.029052E-438.1577459
TAGTACT3250.037.924474
CGAAAGC6650.037.069033
CCGGATA3050.035.6556145
GCGAAAG7450.034.0630382
CTAGTAC3950.033.039193
TAGGCCG900.00362789932.2234085
GTATTAC2802.6193447E-1028.4913061
TCTAGTA5050.025.8435842
CAGTGCG5900.025.8049839
CTATACC2202.228708E-523.069034
GTATATG1902.1887929E-422.9021031
GTATATA7400.022.5410331
GTGCGTA1650.002596341821.9767651
ATATGCG2003.1028254E-421.7508016
GTATTAT5350.021.6892191
TATATAC9050.021.6306293
Produced by FastQC (version 0.11.5)