FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347057
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG24940.18514435543559032No Hit
ATCTATATGTGGATAGTCTTTTTTTAAGTCGGCTCCATGCCCAGCGTGGA24570.18239762682648172No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT24080.17876006731712168No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC20070.14899146806705282No Hit
GTGATGGAGGGACCCAGACAAGATGACCGGAGCCCCACCCAGCCTTGCTG19010.14112246178149848No Hit
CCTCTATGATGCGCAGCAGGGCCTTGACGCCGGGTCTCCACAGGTGGCGC17590.13058096279518983No Hit
TTATTATTGGGCTCAGGTGATGGCTGTGTGAAGATTCACTACTCCATTCC16870.1252359773936812No Hit
AACCTGAACCTACCTATTTTTTTTTAAGTGCTTCAAAGAAATGTCTCCCT15150.11246740115674393No Hit
TAATAGGACTTCATGACTCTATGTCTGCAAAGAACATGCACCATGAATAC14460.10734512348029815No Hit
CATATGGGCCACAAATTTCAAGTTGTATAGCTTGAATAAGGGAAGCAATT14350.10652852848840101No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGAGGAGGAAGTAGGCTCC14260.10586040531321242No Hit
TACCAAGACTTACTTGATTGGAATACCATTTTAAAAACACTCTGGGTAGG14170.10519228213802387No Hit
GTGCTGGAGCAGGTGCAGGTGCTGGAACAGGTGCAAGAGCAGGAACAGGA13900.10318791261245812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6450.0106.8297651
GGCGCCG301.5997188E-596.66598145
GGTAAGG5000.082.661682
GAGCGAA1800.064.47271
AGACTTT3350.054.1081056
CGTTGTA953.1397576E-853.4226538
CGAAAGC13250.053.0829733
ATTCGAC553.2124444E-452.7503851
TAAGGGT8450.052.340794
CGACACT604.944061E-448.3365754
GAACGTC450.009638716548.334788
TCGTTGT1056.9072485E-848.3347787
GAATGGT604.945871E-448.33299145
CTAATGG3100.046.7947041
ACCATAC855.328685E-542.6657561
ATTCGCC855.341541E-542.648347
GCGAAAG16950.041.4955372
TAGGGCG700.001056423241.431354
ACATCGA951.0286746E-438.1604546
TCGACAC800.00203545336.2551233
Produced by FastQC (version 0.11.5)