FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196639
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA13350.11156246787878382No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA12730.10638128959527476No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC12490.10437567219520674No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT12230.1022029200117997No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC203.8663426E-4108.749319
AGCGAAA3100.063.176441
TCCGGAC350.003565583462.147663
GCGAAAG4450.043.992162
CGAAAGC4500.043.503363
ATTACGC700.001056322841.431772
ATAGTAC4650.037.422243
GTCTAGT5000.034.8172381
GTACCTA3050.030.9040246
TACGCCT1000.00607263729.0022414
TTACGCC1000.00607263729.0022413
GATTACG1050.00769043827.6327291
GATTAGC3057.075869E-1026.1604921
GCATTAA5300.026.003441
GTAGAAA8500.025.6009081
TAGCGTT1701.0315028E-425.588074145
GAGCTAT7400.025.4749416
TATAGTA5750.025.2193372
CAATAGG7700.023.550621
TAGTACC3753.074092E-1023.2017924
Produced by FastQC (version 0.11.5)