FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905517
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT26010.13649838862628882No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC24220.1271046125539683No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA21200.11125589538167331No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG20580.10800218523371871No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT20550.10784474764591447No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA19410.10186211930935278No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA19310.10133732735000528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5100.052.63311
GCGAAAG6950.041.7544331
CGAAAGC7450.037.9633642
TATACGT2850.033.0774544
GTAGAAA14550.031.9112911
GCTATTG10600.027.3602038
TAGTACT11400.026.0803014
CTATTGA10700.025.7472489
GTATACG2901.1888915E-825.0061593
CAAGACG5250.024.856743145
GTTATCG1801.521157E-424.1662779
TAACGCA1550.001806221323.393472
GAGCGAA4103.8198777E-1123.0031281
GCCCAGA20950.022.509031
GTGATCA11400.022.2584139
TAAACCG2303.138613E-522.0700745
AGTAGAA16450.022.0511631
TTAGTAC9500.021.3736863
TTAGAGC14700.021.212713
GAGCTAT13450.021.0269326
Produced by FastQC (version 0.11.5)