FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786480
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG20890.11693385876136314No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC19140.10713805920021494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.054.808021
CGAAAGC4850.050.830033
GCGAAAG5900.041.785182
GTAGAAA14300.031.9581971
AGCTATC11350.031.9380749
CGGTTCG1902.0681E-730.5431731
CGATAGC950.00472846330.526073145
CTATACG2903.929017E-1027.5151841
AGTAGAA14500.027.5151841
CCATCGG3800.026.7103149
GAGCTAT13600.026.6549478
TAAACCG1958.754356E-626.027625
TGGTGCG4600.025.21798
TAGTACT10600.023.94054
TATACGT4900.023.6752684
GTGATCA14600.022.8422859
CAATTCG2252.6532141E-522.5553769
ACCGTGC8400.022.4409268
ATGAACG3304.6913556E-821.9713696
TAAGCGC1700.00309337521.3239578
Produced by FastQC (version 0.11.5)