FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037312
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT52640.5074654491609082No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA24990.24091112413622903No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT24020.231560032082922No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA20360.19627653010858837No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG20140.194155663869694No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT18600.17930960019743336No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA17390.1676448358835143No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA16820.1621498642645607No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG16330.1574261167324778No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG15930.15356999629812437No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT15200.14653257650542942No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14960.14421890424481737No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC14680.14151961994076998No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC13770.132746945952616No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC13580.13091528874629813No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT13430.1294692435834156No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA12850.12387786895360316No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT12730.12272103282329715No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT12680.12223901776900296No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA12030.11597282206317867No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG11790.11365914980256664No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC11550.11134547754195459No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG11370.10961022334649556No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC11240.1083569842053307No Hit
CCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAGCAGAGACAGAGG11060.10662173000987167No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG10940.10546489387956565No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC10800.10411525172754196No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC10800.10411525172754196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1051.6370905E-1162.145463
AGCGAAA2300.059.9053651
TACTTCG450.00963764248.3353585
GACAGTG19750.046.2551087
GGACAGT20600.045.050436
CGAAAGC3200.043.0486763
TATACGT1601.0095391E-940.782964
GTAGAAA7800.039.9773331
GCGAAAG3450.039.9311332
CAAGGAC20650.039.6747863
AGTAGAA7950.039.223041
ACAAGGA21400.037.6085322
AACAAGG23300.037.0365871
AGGACAG26400.036.8007855
CGGAGTA850.002742117734.117439
ACAGTGG29650.033.7434658
CGAAGTA1352.1416647E-532.223575
TAACGAC1153.1614036E-431.523063
GTGATCA10250.031.1217569
AAGGACA26800.031.1113784
Produced by FastQC (version 0.11.5)