FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219976
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.032.453661
CGAAAGC4250.032.419232
GCGAAAG4800.028.7151241
TACTCCG1100.00966154726.369715
TATACAG7450.024.3344655
GTATAAA7050.023.6666641
TTATACT8650.023.4736184
TACACCG3203.3789547E-822.661475
GTATATA14550.022.4361251
TATACTG8850.022.1237415
ATTCGAC2303.1282434E-522.078451
CCTTATA7750.021.5210862
ACACCGC4109.367795E-1021.2244016
GTATAAG5002.5465852E-1120.312171
GTATAGG3751.7975981E-719.3449271
TATATAC14300.019.2701743
TCGACAC2659.335302E-519.1553553
CAATTCG3051.27549065E-519.01619
ACCTATC6151.8189894E-1218.8614948
TAATACC7400.018.6191544
Produced by FastQC (version 0.11.5)