FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_D09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_D09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311955
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT16600.12652873002503895No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA15910.12126940329508253No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG15650.11928762800553372No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC15600.11890651737292818No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA15590.11883029524640708No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13930.10617742224390317No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG13790.10511031247260767No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC13120.10000342999569344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2900.062.511093
CCTTATA5600.045.3274542
AGCGAAA4200.043.178881
TTATACT7200.040.2849244
GCGAAAG4900.037.0020032
CAATTCG3850.035.7787489
GTAGAAA8400.033.6795271
TAGACTA1102.4340252E-432.9603925
TCATACG1352.1401098E-532.227943
GACAGTA4050.032.2254877
TATATTC5200.032.0729983
AGGTCGT3200.031.7243796
GTGTAGG1402.7368565E-531.0887931
TATACTC1952.6043017E-729.7488674
GTATTAG1000.006058578429.0162071
CGTCTAG1000.006058578429.0162071
AGCGTGC1255.1551015E-428.99962145
ACAGCGA2553.0213414E-928.4342528
GTATAAT1100.00964635926.3783681
GTAATAC4400.026.3683153
Produced by FastQC (version 0.11.5)