FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951384
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC22460.23607712553501006No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA19900.20916895806530278No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA14990.1575599337386376No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG14550.15293509245478165No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT13090.13758902819471425No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC12710.13359484708592956No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT12250.12875978574371652No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT12250.12875978574371652No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC12000.1261320350142529No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA10910.11467504183379161No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA10300.10826333005390042No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA10030.10542535926607974No Hit
GATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGC9730.10227205839072341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1700.068.26381
GCGAAAG2100.055.2379342
CGAAAGC2700.042.962843
GTAGAAA8450.036.90891
ACCGTGC5000.036.2498978
TACGGTG800.00203659436.2498937
CTATTGA6350.034.251879
GCATTAA4700.033.9503441
TACTCCG900.00362819532.2221267
GCTATTG6900.031.5216488
CTATACG1703.0017254E-629.8654141
ATAGTAC3153.45608E-1127.6189693
ATGAACG1050.007708374427.6189676
TTACGAA1606.8279274E-527.1874224
AGTAGAA8900.026.8932531
CCATCGG1358.0825575E-426.8517749
TTAGAGC8400.026.7558753
ATCCCGG1100.00965168226.374651
TCCACCG6650.026.1653375
CCGTGCT7850.025.8597989
Produced by FastQC (version 0.11.5)