FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588711
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA26760.45455240347131276No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC15490.2631172171065259No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG12320.20927076273417689No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC10920.18548999424165677No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG10160.17258043420286015No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG8730.14829007781407175No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA8730.14829007781407175No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG7980.13555038040736456No Hit
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT7850.13334216619020198No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA7840.13317230355811255No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT7550.12824628722751913No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC6570.11159974928275504No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT6570.11159974928275504No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG6540.11109016138648674No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA6090.10344634294246244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1550.056.142323
AGCGAAA1600.049.8724981
GTCCGGA657.322502E-444.6297382
AGTGATC12200.042.1918568
ACCATCG4550.039.834448
CCATCGG4650.038.9777879
GTGATCA12800.038.5149279
CCTATCA8900.036.6784441
AAGTGAT14900.036.4957287
GTGATCC9450.035.2903639
GGTGATC9050.035.247988
CTATCAG9300.035.091932
CAGTCGA850.002741318234.1170279
GTAGAAA4550.033.4808351
ATGAACG3250.033.469466
AGGTGAT10200.033.4090967
GCGAAAG2404.3655746E-1133.2398572
GTATAGT1102.4267098E-432.973551
CTGAAGT15950.032.735024
TGAAGTG16100.032.430035
Produced by FastQC (version 0.11.5)