FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027992
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3250.058.0222051
CGAAAGC3500.053.862043
TACCTAT5550.032.6590357
GTAATAC5750.030.263623
GCGAAAG6700.028.1368872
ATACCTA5200.027.887156
GTATAGT3950.027.5421871
TAATACC5900.027.0363564
CAATTCG1851.7348375E-623.5133639
GTATTAA6650.022.9035031
TATATAC10950.022.5134623
GTATTAC6650.021.812861
TATACAG7350.021.7026525
TATTACA8700.021.6686382
TATAATA5101.8189894E-1221.3254682
ACCTATC6800.021.3233938
TATATTC5850.021.0702923
GTATATA10050.020.9284081
AATACCT8100.020.5882915
TATACCA5002.5465852E-1120.3018465
Produced by FastQC (version 0.11.5)