FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577702
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1950.081.815041
CGAAAGC2650.060.198313
TATATAT3250.042.3913962
CGTATTG700.001056338241.428039
CCTCGCG907.479091E-540.27725145
ATATATA3150.036.8344761
TACGCAG1001.3894134E-436.2558025
GGACGTA800.002034306336.25586
GACGTAT800.00203517136.2526637
GTATAAG1551.4607722E-632.7500081
GTTACGC1153.1574946E-431.5267833
CTATTAA2807.2759576E-1231.0790921
GCGAAAG5150.030.975832
TTACGCA1255.1463564E-429.0046394
TAGTAGC1000.006070183629.002137
GGGTGAT1804.692769E-628.1965147
TACTATA1306.47355E-427.8890782
GTACTAT3153.45608E-1127.6258561
TTGATAC1606.8158E-527.1918492
GTATATA3306.184564E-1126.3701361
Produced by FastQC (version 0.11.5)