FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37224
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAG6861.842897055663013No Hit
TCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAG3911.0503975929507845No Hit
GTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTG3150.8462282398452611No Hit
GTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCC2810.7548893187191059No Hit
AAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAG2410.6474317644530411No Hit
GTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCC2010.5399742101869762No Hit
GGTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGC1960.526542015903718No Hit
GTACATGGACACGGCATTCTGGGAAAAGTCAAAGAGAACACGGAAAACTT1910.5131098216204599No Hit
GTCCAGGACCTTTTCAAGGCTGTCTCCATGGCAACGACAATCTTGTGAAC1880.5050505050505051No Hit
ATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCC1800.4835589941972921No Hit
TCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAG1780.47818611648398884No Hit
GTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAG1690.4540081667741242No Hit
AGCAGGTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGA1660.4459488502041693No Hit
ATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTG1590.42714377820760796No Hit
GGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTCAGTTC1540.41371158392434987No Hit
TTTCAAGGCTGTCTCCATGGCAACGACAATCTTGTGAACTGAGAATCTGT1510.4056522673543951No Hit
GTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTT1500.40296582849774343No Hit
GGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACT1490.4002793896410918No Hit
ATGCTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAAC1490.4002793896410918No Hit
AAGCAGGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAA1480.3975929507844401No Hit
CCCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAGATTGTCGTTGCCA1370.3680421233612723No Hit
TATGTATCCAGTGAGATGCGCTCCGGCCCCATCCTGATTCTCCTGAAGGG1360.3653556845046207No Hit
GTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTG1320.3546099290780142No Hit
GTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAG1320.3546099290780142No Hit
CCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTCTCTGTCCAGGAC1290.3465506125080593No Hit
ATGCATATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGT1290.3465506125080593No Hit
GAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTC1270.3411777347947561No Hit
GCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCT1260.33849129593810445No Hit
CCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCATGG1260.33849129593810445No Hit
AATAAGTAGTGTGTGAAAGAAAAACGAGAAAGAGAGAGAGAGAGAGAGAG1240.3331184182248012No Hit
GTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGT1230.3304319793681496No Hit
GAAATAAGTAGTGTGTGAAAGAAAAACGAGAAAGAGAGAGAGAGAGAGAG1210.3250591016548463No Hit
TGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGC1180.31699978508489146No Hit
GCATTGAACATTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCG1160.31162690737158827No Hit
GGTGAATGCATATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAG1120.3008811519449818No Hit
TATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATG1110.2981947130883301No Hit
CTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGT1090.2928218353750268No Hit
GGGTATGACTGAGTGACTAATTGGCTTGTCTGCCTGATCTGGCGCATACT1070.2874489576617236No Hit
GAACATTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTA1030.27670320223511713No Hit
AAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTTTGTTGTT1010.2713303245218139No Hit
GTGTTAAACTTTTGTTGTTTTCGTTGGGTTGCCGTGGAGGAGGGAAGTCA1010.2713303245218139No Hit
GTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGA920.24715237481194927No Hit
ATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTG920.24715237481194927No Hit
CACCTGGACGACATCCTGCCTCTCTTGTTGCAGGCTAGCACAGACAGCCA900.24177949709864605No Hit
GGATGGGGGTCGTGGGGTGGTGAATGCATATCTGTAATATGTACGTGTGT900.24177949709864605No Hit
AAGCAGGACAGACACAGGGACAGACGTGATCATATGTATCCAGTGAGATG900.24177949709864605No Hit
AAGCAGGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATG890.23909305824199442No Hit
CTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTC890.23909305824199442No Hit
GCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATT880.2364066193853428No Hit
CCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAACTTTAG880.2364066193853428No Hit
CAGGAAGGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGT840.2256608639587363No Hit
GATCATATGTATCCAGTGAGATGCGCTCCGGCCCCATCCTGATTCTCCTG830.22297442510208465No Hit
CACCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCAT820.22028798624543305No Hit
GCAGGGGCCAGTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAA820.22028798624543305No Hit
TTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCATATTAGTGAGG820.22028798624543305No Hit
CAGGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGC770.20685579196217493No Hit
ATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTT740.19879647539222006No Hit
GCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAACTTTAGTAAGCC730.19611003653556844No Hit
GAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTT730.19611003653556844No Hit
GGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCC720.19342359767891684No Hit
CCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAGATTGTCGTTGCCAT710.1907371588222652No Hit
CACCCACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGT710.1907371588222652No Hit
CACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCCCC700.1880507199656136No Hit
GTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAAC690.18536428110896197No Hit
GGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAG690.18536428110896197No Hit
AGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACC690.18536428110896197No Hit
ATCTTCACCTGAGCAGGGGTTCACGTTGCTCGGTAGGTCTAACCTACCTT690.18536428110896197No Hit
GAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGA680.18267784225231035No Hit
GGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTCTCTGTC670.17999140339565872No Hit
CATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCATATTAGTGA670.17999140339565872No Hit
CTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGT670.17999140339565872No Hit
CCCACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAA640.17193208682570385No Hit
CCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAA640.17193208682570385No Hit
GGACAGACACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTC640.17193208682570385No Hit
AGCGAAAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAAC620.1665592091124006No Hit
CTGCAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGG620.1665592091124006No Hit
GCAGGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGC610.16387277025574898No Hit
TCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGGCATTCTGGGAA610.16387277025574898No Hit
AAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGC580.15581345368579413No Hit
GTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCA580.15581345368579413No Hit
CTGTTGGGCTTGGCTGTGCTGCCGCCTGTTATTTATTGCAGAGCTGGCTG570.15312701482914248No Hit
CAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCGATCTTCAC570.15312701482914248No Hit
CAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTGACTTTTCCCAGAA570.15312701482914248No Hit
GGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAA570.15312701482914248No Hit
GTCAAAGAGAACACGGAAAACTTTTAGTCAGGACTACTGCGATGTTGTAC560.1504405759724909No Hit
GTGCATACTGGTTAGATTGAGTGCAGGTGTAGTGGTAACTGGGTGGGAAA560.1504405759724909No Hit
CACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGTGACTAA560.1504405759724909No Hit
GGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACAT560.1504405759724909No Hit
CATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGT550.14775413711583923No Hit
GGCAAAGGCGTGGGATGGGGGTCGTGGGGTGGTGAATGCATATCTGTAAT550.14775413711583923No Hit
GACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACATT550.14775413711583923No Hit
GACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCCCCATCCTGATTC550.14775413711583923No Hit
GATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACA530.142381259402536No Hit
AGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTG530.142381259402536No Hit
GTTCACAAGATTGTCGTTGCCATGGAGACAGCCTTGAAAAGGTCCTGGAC530.142381259402536No Hit
GCCCGTGGGTATGACTGAGTGACTAATTGGCTTGTCTGCCTGATCTGGCG530.142381259402536No Hit
GTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAG520.1396948205458844No Hit
CAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATT520.1396948205458844No Hit
GCAGTACATGGACACGGCATTCTGGGAAAAGTCAAAGAGAACACGGAAAA520.1396948205458844No Hit
AGCACACACAGCCCGTGGGTATGACTGAGTGACTAATTGGCTTGTCTGCC520.1396948205458844No Hit
CTTTTGTTGTTTTCGTTGGGTTGCCGTGGAGGAGGGAAGTCAAATTTGAA510.13700838168923274No Hit
GTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGCGTGTCCT510.13700838168923274No Hit
TCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTG510.13700838168923274No Hit
GTCCTGGACAGAGACAACAATCCACAGGTGTCTGTGGAAAGGGGGAAGGG510.13700838168923274No Hit
CTTGTGAACTGAGAATCTGTCTGATTGGGCCTAGGGGAAGGCAACAATCC510.13700838168923274No Hit
GGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGC500.13432194283258114No Hit
GGCCAGTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAA500.13432194283258114No Hit
CACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGG490.1316355039759295No Hit
GCGGTAAGCGATCTTCACCTGAGCAGGGGTTCACGTTGCTCGGTAGGTCT490.1316355039759295No Hit
GTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTGAC490.1316355039759295No Hit
GCCTTGAAAAGGTCCTGGACAGAGACAACAATCCACAGGTGTCTGTGGAA490.1316355039759295No Hit
GCAGGAAGGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTG480.1289490651192779No Hit
CTCCATGGCAACGACAATCTTGTGAACTGAGAATCTGTCTGATTGGGCCT480.1289490651192779No Hit
ACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCG480.1289490651192779No Hit
CTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCGATCTTCACCTGAGCA480.1289490651192779No Hit
GTCATGCTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTC480.1289490651192779No Hit
TCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCATGGCAACGACAATCTTG470.12626262626262627No Hit
ATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCAT470.12626262626262627No Hit
GGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTTTGTTGTTTTCGT470.12626262626262627No Hit
GGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCCCCATCCT460.12357618740597463No Hit
GCCATGGAGACAGCCTTGAAAAGGTCCTGGACAGAGACAACAATCCACAG460.12357618740597463No Hit
CTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGGCATTCTGGGAAA460.12357618740597463No Hit
CTCCGGGACTTACGCAACCCTTCAGGAGAATCAGGATGGGGCCGGAGCGC460.12357618740597463No Hit
TTGTAAGTGTTAAACTTTTGTTGTTTTCGTTGGGTTGCCGTGGAGGAGGG460.12357618740597463No Hit
GTTATTTATTGCAGAGCTGGCTGGGAGCTCAGCATGGTGTCTGCAAACTG450.12088974854932302No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG440.1182033096926714No Hit
CGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACA440.1182033096926714No Hit
CACACACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGT440.1182033096926714No Hit
TTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTAGTCCT430.11551687083601977No Hit
GTCCCGGAGTATGCGCCAGATCAGGCAGACAAGCCAATTAGTCACTCAGT430.11551687083601977No Hit
ACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTGACTTTTC430.11551687083601977No Hit
CCACAGACACCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTG420.11283043197936815No Hit
AGCGAAAGCAGCGGAGTTCATCGACACACACCTGGACGACATCCTGCCTC410.11014399312271653No Hit
CATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAG410.11014399312271653No Hit
GACACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCC410.11014399312271653No Hit
AAAGCAGGCAGCTGTTGGGCTTGGCTGTGCTGCCGCCTGTTATTTATTGC410.11014399312271653No Hit
ATTTATTGCAGAGCTGGCTGGGAGCTCAGCATGGTGTCTGCAAACTGGGT400.1074575542660649No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG400.1074575542660649No Hit
GACAGAGAGAGAGAGAGAGAGAGGAGAAGTAGCAGAGGGAGAGAGAGAGA400.1074575542660649No Hit
CCACTACACCTGCACTCAATCTAACCAGTATGCACAGCCTGTTTCTCTCC400.1074575542660649No Hit
CACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGTGACT400.1074575542660649No Hit
CTCTTTGACTTTTCCCAGAATGCCGTGTCCATGTACTGCCGCCTGGGTTT390.10477111540941328No Hit
CTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGA390.10477111540941328No Hit
GCCGTGGAGGAGGGAAGTCAAATTTGAAAGTGACGGCAGCTGTGGGCTCT390.10477111540941328No Hit
CCTCATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTC390.10477111540941328No Hit
CTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTCTCTG390.10477111540941328No Hit
ACAGACACCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTC390.10477111540941328No Hit
AGCGAAAGCAGGGGCCAGTCCGCCCCTGGCCCCAGCGCGTCCGACACACC380.10208467655276166No Hit
ATCCACAGGTGTCTGTGGAAAGGGGGAAGGGAATGCCAGAGTCTCACTGC380.10208467655276166No Hit
GTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCATGGCAA380.10208467655276166No Hit
GTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGCGTGTCCTTC380.10208467655276166No Hit
CTCCTGAAGGGTTGCGTAAGTCCCGGAGTATGCGCCAGATCAGGCAGACA380.10208467655276166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATA100.007063639144.999982
GCAGGTA351.8517312E-9124.285712
AGCAGGT458.185452E-11112.777781
AGCGAAA451.0628355E-896.6666641
GAAATAA406.0017555E-790.624991
CAGGTAC406.0017555E-790.624993
GTTCTTG502.2087988E-887.09
GCAGGCA259.315354E-487.04
GCAGCCT259.315354E-487.08
GGTTCTT607.820745E-872.58
CGAAAGC702.7139322E-972.52
AGGCAGC300.001921073272.56
GGCAGCC300.001921073272.57
AAGCAGG2000.068.8751
TCTGGAC1500.067.666673
TTCTGGA1500.067.666672
CTGGACT1500.067.666674
TGGACTG1500.067.666675
GGGTTCT651.3614226E-766.923077
GGACTGA1550.065.483876
Produced by FastQC (version 0.11.5)