FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG7450.10562431769526322No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA100.007089189145.008448
AGCGAAA1650.065.940981
CGAAAGC2100.055.2452283
GCGAAAG2200.052.7340772
ACCGTCA657.333005E-444.6179778
GACCGTC1105.2599935E-639.5477527
TGTACCG1209.551399E-636.2546775
CGAGGCG1604.5869456E-836.24954145
GGACCGT1458.6848195E-735.004526
ATGCGAC950.004725015330.5280937
GTAGAAA4300.030.3635161
AGACCGC1453.4892542E-529.99962145
CAATAGG3700.029.4061131
TGCGACA1000.006071532629.0016888
AGCTATC3500.028.9996329
ACCGTGC3950.027.5332498
ATAAGAC2156.113387E-726.9802253
ATAGGAG4800.025.6803973
TAACGTG4001.8189894E-1225.3782775
GAGCTAT4051.8189894E-1225.0631898
Produced by FastQC (version 0.11.5)