FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808066
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA46600.5766855677630293No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT33230.41122878576749916No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG28670.35479775166879934No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA28300.35021891776166797No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT24610.3045543309581148No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT21870.27064620959179075No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA19450.24069816079379655No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA17090.21149262560236415No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG16970.2100075983892405No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC16580.2051812599465885No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC16480.20394373726898546No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG16370.20258246232362206No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG15640.1935485467771197No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15420.19082599688639296No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA15420.19082599688639296No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC15190.18797969472790588No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC15160.18760843792462498No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14930.1847621357661379No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG14460.1789457791814035No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC14320.1772132474327592No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14260.17647073382619738No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG14180.1754807156841149No Hit
CATCATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACA13700.1695406068316202No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13450.1664468001376125No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13290.16446676385344763No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG13200.16335299344360485No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG13170.16298173664032392No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT13110.1622392230337621No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG13100.1621154707660018No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAT12920.15988792994631626No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG12920.15988792994631626No Hit
GTTCATTGATGCTTAATGCTGGGCAAAATGAGAAATCCTCTCAGGACAGC12820.1586504072687132No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT12770.15803164592991167No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC12730.15753663685887043No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA12520.15493783923590398No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT12420.1537003165583009No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12230.1513490234708551No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11980.14825521677684744No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA11800.1460276759571619No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11750.1454089146183604No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT11720.14503765781507946No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT11610.14367638286971607No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11520.1425626124598733No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11420.14132508978227026No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11310.1399638148369069No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG11280.13959255803362597No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG11170.13823128308826257No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA10780.13340494464561065No Hit
GCTTAATGCTGGGCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCACC10740.1329099355745694No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10730.13278618330680908No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA10700.13241492650352818No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA10420.1289498630062396No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10420.1289498630062396No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT10260.12696982672207469No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA10130.12536104724119068No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT10120.1252372949734304No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10100.12498979043790977No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC9940.12300975415374486No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG9930.12288600188598456No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT9870.12214348827942273No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA9480.11731714983677076No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA9460.11706964530125014No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA9420.11657463623020892No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA9200.11385208633948217No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA9190.11372833407172185No Hit
CCATATGGCCATAATCAAAAAGTACACATCATGGCAATGAGATACCCAAT9030.11174829778755695No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG8940.11063452737771419No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT8910.11026327057443329No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA8870.10976826150339206No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT8860.10964450923563174No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA8770.10853073882578898No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA8670.1072932161481859No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA8600.10642695027386377No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA8590.10630319800610345No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG8560.10593194120282254No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA8380.10370440038313701No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA8370.10358064811537672No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT8320.10296188677657518No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC8260.10221937317001334No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG8250.10209562090225303No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG8110.10036308915360875No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACG100.0070829242145.052631
CTCGGGG256.464392E-6115.99901145
AGTAGAA4450.0112.456521
GCAGGTA2450.0106.5560762
CAGGTAC2650.098.508013
GAATAAC1000.087.031561
TAACTGA1050.082.866694
AAATATA4150.082.11795
CGAAAGC1250.081.214383
AGGTACT3400.078.906154
AGCGAAA1300.078.1052551
GACAGTA2800.077.682727
GGTACTG3400.076.773555
GTAGAAA6800.073.5837552
ACTGATC3550.073.52058
GCGAAAG1450.070.016732
GTACTGA4150.064.6466
ATAACTG1350.064.4558643
ACAATTC8550.064.443898
TTTAAAC8350.064.262873
Produced by FastQC (version 0.11.5)