FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569327
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA7470.13120754856172287No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA6090.10696840304429615No Hit
GCCTAAGAGAGAGGATTTCTGGGTCTTGGGAGAGGCGACCTTCCCTGGGC5770.10134773161996533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2150.053.953169
CGAAAGC1203.614332E-948.3372843
TACACGA450.00963390848.3372845
AGCGAAA1255.178663E-946.411951
CGTTATA907.474772E-540.281072
TTATACT5900.038.0963364
AGTAGAA5050.037.3363461
TCGTTAT800.00203330836.2593351
GTAGAAA5400.036.2529642
TATACTG7500.035.7695925
GCGAAAG1656.004666E-835.154392
CCTTATA5250.034.526632
AGTGATC4150.033.1954888
GTTATAC2051.1359589E-831.8318713
ATGAACG1204.0539296E-430.2108046
ACAATTC4100.030.0634358
GGATAGC1703.006795E-629.8553855
CCCTTAT6700.028.1415711
GTGATCA5000.027.5498339
CAAGACG1606.823336E-527.187336145
Produced by FastQC (version 0.11.5)