FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369777
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA11100.3001809198516944No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC8550.23122043826414299No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC6960.18822154974484623No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG6700.18119028495552725No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC6140.1660460223323787No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT6070.16415298950448515No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT6050.16361212298222982No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA6020.1628008231988469No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC5920.16009649058757036No Hit
GTTATAATCTCTCCTTTCCCTCACTCCCCTTTTCCCTCCTTCTGAACTCA5660.15306522579825138No Hit
GGTGTAGGGTTTGTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCAT5550.1500904599258472No Hit
GTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCATACGTTTATTTTG5320.1438704949199112No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA4920.13305316447480509No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC4600.12439930011872022No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG4590.12412886685759254No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG4520.12223583402969898No Hit
AACTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAA4450.12034280120180542No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG4440.12007236794067777No Hit
GAAGTGGGATCATACGTTTATTTTGCACTTCATTTTTCCCACTAAATAAT4390.1187202016350395No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC4300.11628630228489062No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA4290.11601586902376297No Hit
GTATAATACAGTGATTAGACAAGTCTATAAGTTATGCTGTGCTCAGGAAA4290.11601586902376297No Hit
CTTTGAAGATGTTATAAAAGAAAAAGTTATAATCTCTCCTTTCCCTCACT4260.11520456924038No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG4240.1146637027181247No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA4170.11277066989023114No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT4170.11277066989023114No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT4150.11222980336797583No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG4140.11195937010684819No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC4060.10979590401782698No Hit
AAGTAATATTTTCCCCCTTCTTAAATAAAAAGCATAAATCCTTAGGAATG4040.10925503749557165No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG4000.10817330445106103No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT3960.10709157140655044No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA3890.10519853857865685No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT3870.10465767205640156No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG3840.10384637227301861No Hit
CTATTAACGTGTTCTATTTGACCAAAAAAGAAGTTACAGTTAATAGAGTT3800.102764639228508No Hit
ATGTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAAC3800.102764639228508No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA3720.10060117313948677No Hit
GCGTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTT3720.10060117313948677No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG3700.10006030661723146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA608.221832E-1084.6620561
GTAGAAA2750.065.9704361
ATGAACG1100.065.925816
TATATAC350.00356114562.1586233
GCGAAAG954.5292836E-1061.0763852
TAGACAG400.00604181454.3887985
CGAAAGC953.1259333E-853.4346053
TAGAAAC4000.047.1433332
AGTAGAA1950.044.656911
GTGATCA4950.039.5447929
CGACCAA750.001480328938.676485
CCGACCA750.001480328938.676484
TAACGAC1157.1181057E-637.8356863
TTAACGA1209.52212E-636.2641032
ATAAGAG800.002032425336.2591973
CTGAAGT5650.035.938324
CTTATGA2252.0008883E-1135.453443
CTTAACG1251.2549117E-534.832391
TAGTCCT1051.848704E-434.532574
TTAGAGT850.00273635534.1263054
Produced by FastQC (version 0.11.5)