FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679522
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACATCTGAGGGAAAAAACTGCAAAAATGTCCCCAAATCTGTGGAAA26290.3868896076948207No Hit
GAGTATGAGAAGTATATGAGAAAATGATGCAGAATATACATCTGAGGGAA16290.23972733774623925No Hit
GTATATGAGAAAATGATGCAGAATATACATCTGAGGGAAAAAACTGCAAA16030.23590111872757616No Hit
GAATATACATCTGAGGGAAAAAACTGCAAAAATGTCCCCAAATCTGTGGA13190.194107034062179No Hit
CTTTAGTATTTCTTGTACTTCAGATCTCTAAGCAGTTACTTCAGACGACT13100.19278257363264178No Hit
TCTCTAAGCAGTTACTTCAGACGACTTTCTCTTTTCCTCTTAGAACTTTA13030.1917524377430017No Hit
GTATTTATGTTGTTAGGTCAGCCAAGGATTTGAATGTAAATCTTTGTACC11210.16496890461235986No Hit
GTATTAGAAAATGTACGAGATGTGCTGTAGAAGTTTTGGTTTCTCTCTAT11070.16290863283307974No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA10310.15172430031698753No Hit
GAATATATGCAAATCCAATGATCTCAGTAATCTCTAAGCAGGAAAAATAC9970.14672078313873574No Hit
TTCTTGTACTTCAGATCTCTAAGCAGTTACTTCAGACGACTTTCTCTTTT9810.14436618681955846No Hit
CTATAGAGTTTTGTTTTTTGCTCTGGTAAGTAGTTACATTATTGGTCAAT9380.13803820921176943No Hit
CTTCTATAGTGCTTATTGTGTGCTAGGCACTGTTCTAAGCAGTTGTACAC9010.13259320522367193No Hit
GCCTAGGGAGGGGGGACAGCACCGGTTACCTGGCAAGCAGAGGTGGCAGC8800.12950279755475172No Hit
TCCTTATAGCTACTGTAATTTTTAAACTTTCAAAGGATATTACCATTCGT8540.1256765785360886No Hit
TCCTAGAGCTGCGTGGGCGCCCCTCCAGGCCCTCTAGCACCTTCCCTCCT8540.1256765785360886No Hit
CTCTAGCACCTTCCCTCCTGCACTTCCCTCTAGATGCTGCGGAGGCAGCC8500.12508792945629427No Hit
CCTTATAGCTACTGTAATTTTTAAACTTTCAAAGGATATTACCATTCGTC8430.12405779356665422No Hit
ATTATAAACTATGGACAAAATTTTTTAAAAACTATTTGAAGGCAAGGAAG8180.12037873681793967No Hit
GGTAAGTAGTTACATTATTGGTCAATTATCTGGAGGCTTGGTTTTATGCT8130.11964292546819676No Hit
ACCCTGCACACAACCCACAGAGTCTTTGGCCTGGAGGCCTCTTGAGAGGG8000.1177298159588652No Hit
GTATATAGTGAAGGACCAGGCTCTGCCCAAGAAACTTTGGTCAAGTTTTC7990.11758265368891661No Hit
GGATATTACCATTCGTCTTTCAATGTTAAAGTAGCAAACACTTCTATAGT7460.1097830533816418No Hit
GCATAAAGAGGGACACCAAAGAGAACATGAAAAGAGGCACAATATCACTT7330.10786994387231023No Hit
CTGTTAGAACAGGTTTGTAGAAAGCCCAAATTATTTACCAAGCATCCCTG7290.10728129479251591No Hit
GTCTTTGGCCTGGCGTTGTGGATCTAGTGCTCATTCAGTCCCCGCAGCAA7230.10639832117282443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC100.007084578145.039613
CTTCGCG100.0070908163144.99692145
AGCGAAA2500.0116.065851
CGAAAGC4100.070.751023
GAGTATG4100.065.4639741
GCGAAAG4500.064.466792
CTAGTAC2250.064.462043
TAGTACT2450.059.195484
GAGCGAA502.0041532E-458.032931
AGTATGA5000.055.1191062
GTATGAG5200.054.389853
CACGAGC553.218606E-452.726147145
TATAAAC3550.051.0702823
ACACCGG450.00963582748.335868
TGTTGAC1002.7263304E-643.515082
CATAATA1703.8198777E-1142.6618462
AATAGTG1703.8198777E-1142.6555675
TAGGACG2250.041.8972474
ATAGGAC2600.041.838353
GTATTGA700.00105345641.4520871
Produced by FastQC (version 0.11.5)