FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663133
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT7510.11325028312570783No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG7100.10706751134387824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.045.8232421
CGAAAGC2400.039.282013
TATACTT5250.035.9149785
CAATTCG2551.8189894E-1234.117069
GCGAAAG2653.6379788E-1232.8420072
TATATAC8100.029.545443
TATACAG5200.029.2871785
CAGCTCG4350.028.3328489
CCGTACT2354.2811735E-827.7738594
GTATAAA4550.027.1078871
CTAGTAC1100.00965587826.3711383
GTAGAAA8250.025.503641
CCGGCGT1450.001226129924.99957145
ATACTTA6150.024.763146
TATAACA2351.3251465E-624.6897372
TAGAAAC8600.024.4564742
ATATACT5050.024.4128844
GAGTTAT1801.5189321E-424.1680747
AAGGTCG3102.4152541E-823.3866969
ATATATA5900.023.3558642
Produced by FastQC (version 0.11.5)